文献类型: 外文期刊
作者: Liu, G. -F. 1 ; Fang, W-N 1 ; Lin, H. -C. 1 ; Wang, X. -F. 4 ; Fu, J-L 1 ; Wang, A. -G. 1 ;
作者机构: 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China
2.Minist Agr, Key Lab Anim Genet & Breeding, Beijing 100193, Peoples R China
3.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
4.Anim Husb & Vet Serv Stn, Beijing 100107, Peoples R China
关键词: 3-hydroxy-3-methylglutaryl-CoA reductase;cholesterol;mRNA expression;pig
期刊名称:ANIMAL ( 影响因子:3.24; 五年影响因子:3.223 )
ISSN: 1751-7311
年卷期: 2009 年 3 卷 10 期
页码:
收录情况: SCI
摘要: The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. To study the expression of HMGR and corresponding cholesterol content in liver, adipose and muscle, six Chinese local breed (Huai pig) and Landrace pigs were selected. The results indicated that significant differences of cholesterol content in adipose (P < 0.01), liver (P < 0.05) and muscle (P < 0.01) tissues were detected between pigs of differing genetic backgrounds. H M G R mRNA expression were noted for adipose, liver and muscle of the two vastly differing genetics. Moreover cholesterol content differed (P < 0.01) among tissues across breed. Likewise, HMGR mRNA expression was different between adipose and liver tissues, muscle and liver tissues in both breeds; however, no difference was noted between adipose and muscle tissues. Results from this study indicate that cholesterol content and HMGR mRNA expression are higher in Huai pig tissues suggesting this gene is expressed in a breed- and tissue-dependent manner in pigs. Understanding the causes of variation in HMGR gene expression may provide crucial information about cholesterol biosynthesis.
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