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Effect of SIRT1 on Apoptosis of Bovine Preadipocytes

文献类型: 外文期刊

作者: Liu Xiao-Mu 1 ; Song En-Liang 1 ; Liu Gui-Fen 1 ; Wu Gong-Ying 3 ; Tan Xiu-Wen 1 ; Liu Zhen-Shan 1 ; Wan Fa-Chun 1 ;

作者机构: 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

2.Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China

3.Shandong Vocat Anim Sci & Vet Coll, Weifang 261061, Peoples R China

关键词: SIRT1;nicotinamide;bovine;preadipocytes;apoptosis

期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )

ISSN: 1000-3282

年卷期: 2010 年 37 卷 3 期

页码:

收录情况: SCI

摘要: Sirtuin type 1 (SIRT1), a novel regulatory factor of adipocyte and myocyte, interacts with target the forkhead box O family 1 (FoxO1) to modulate cell proliferation and differentiation, aging, apotosis and metabolism. The effect of nicotinamide (NAM, inhibitor of SIRT1) on the apoptosis of bovine preadipocytes was investigated with Acridine Orange (AO) staining, the flow cytometry detection and quantitative real-time PCR (qPCR). The results showed that NAM inhibited the differentiation of bovine subcutaneous preadipocytes (BSP) and bovine intramuscular preadipocytes (BIP), and their apoptosis rates were higher than control. SIRT1 influenced the apoptosis of bovine preadipocytes by regulating FoxO1 and adipocyte marker genes. The different mechanisms of SIRT1 and its related genes exist in BSP and BIP.

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