文献类型： 外文期刊
作者： Song, Guo Qi 1 ; Li, Meng Jun 1 ; Xiao, Han 1 ; Wang, Xing Jun 1 ; Tang, Rong Hua; Xia, Han 1 ; Zhao, Chuan Zhi 2 ; Bi 1 ;
作者机构： 1.Shandong Acad Agr Sci, High Tech Res Ctr,Minist Agr, Key Lab Genet Improvement Crop Anim & Poultry Sha, Key Lab Crop Genet Improvement & Biotechnol, Jinan 250100, Peoples R China
2.Shandong Acad Agr Sci, High Tech Res Ctr,Minist Agr, Key Lab Genet Improvement Crop Anim & Poultry Sha, Key Lab Crop Genet I
关键词： Arachis hypogaea L.;cultivars;EST-SSR;polymorphism;wild-type peanut
期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）
ISSN： 0717-3458
年卷期： 2010 年 13 卷 3 期
页码：
收录情况： SCI
摘要： Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA (9.0%), CTT/GAA (7.4%), and AAG/TTC (7.3%) repeats. Ninety-four SSR containing ESTs were randomly selected for primer design and synthesis, of which 33 pairs could generate good amplification and were used for polymorphism assessment. Results showed that polymorphism was very low in cultivars, while high level of polymorphism was revealed in wild type peanuts.
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