您好，欢迎访问山东省农业科学院文献资源数据库平台

Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity

文献类型： 外文期刊

作者： Liu, Huaiwei 2 ; Zhang, Bo 1 ; Li, Changsong 1 ; Bao, Xiaoming 2 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China

2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

关键词： Chitosanase;Fusarium solani;RNA silencing;Pathogenicity

期刊名称：CURRENT GENETICS （ 影响因子：3.886； 五年影响因子：3.697 ）

ISSN： 0172-8083

年卷期： 2010 年 56 卷 3 期

页码：

收录情况： SCI

摘要： Chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. The phytopathogenic fungus Fusarium solani produces an extracellular chitosanase, CSN1, the role of which in the physiology and virulence of the fungus remains to be expounded. Here, we studied the expression of the CSN1 gene through gene silencing and examined its effect on fungal pathogenicity. A vector construct encoding a hairpin RNA (hpRNA) of CSN1 was constructed and introduced into the F. solani 0114 strain. The results revealed that majority of the transformants exhibited a significant reduction in chitosanase activity compared with the wild-type strain. Further, transformants with silenced CSN1 exhibited no change in mycelial growth and spore formation. However, pea pod and seedling bioassays indicated that transformants with silenced CSN1 were more virulent compared with the wild-type strain, and in sharp contrast to strains in which overexpression of the CSN1 gene resulted in virulence reduction. Although the mechanism remains unclear, our findings did suggest that F. solani chitosanase has a negative effect on fungal pathogenicity.

  • 相关文献

[1]A highly Conserved Aspartic Acid Residue of the Chitosanase from Bacillus Sp TS Is Involved in the Substrate Binding. Zhou, Zhanping,Liu, Yang,Chang, Zhengying,Ma, Yanhe,Song, Jiangning,Zhou, Zhanping,Liu, Yang,Chang, Zhengying,Ma, Yanhe,Song, Jiangning,Zhao, Shuangzhi,Li, Jian,Song, Jiangning,Song, Jiangning.

[2]PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice. Cheng, Kaihui,Yu, Zhijun,Sun, Weiyang,Xin, Yue,Huang, Jing,Zhang, Kun,Li, Xue,Yang, Songtao,Wang, Tiecheng,Zheng, Xuexing,Wang, Hualei,Qian, Jun,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Zhang, Qianyi,Chen, Hualan,Chai, Hongliang,Hua, Yuping.

[3]Multiple amino acid substitutions involved in the virulence enhancement of an H3N2 avian influenza A virus isolated from wild waterfowl in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Zhao, Chuqi.

[4]Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice. Bi, Yuhai,Lu, Lu,Li, Jing,Zeshan, Basit,Sun, Lei,Liu, Wenjun,Lu, Lu,Yin, Yanbo,Qin, Zhuoming,Zhang, Yi,Gao, Huijie,Liu, Jinhua. 2011

[5]Parasitism of secondary-stage juvenile of Heterodera glycines and four larva stages of Caenorhabditis elegans by Hirsutella spp. Sun, Jingzu,Xie, Hongyan,Liu, Xingzhong,Xiang, Meichun,Xie, Hongyan,Qiu, Junzhi.

[6]Two different genotypes of H1N2 swine influenza virus isolated in northern China and their pathogenicity in animals. Yang, Huanliang,Chen, Yan,Qiao, Chuanling,Xin, Xiaoguang,Bu, Zhigao,Chen, Hualan,Xu, Chuantian,Yan, Minghua.

[7]Identification of Wohlfahrtiimonas chitiniclastica isolated from an infected cow with hoof fetlow, China. Qi, Jing,Li, Lu-lu,Du, Yi-jun,Liu, Yu-qing,Gao, Yang,Zhao, Xiao-min,Wang, Gui-sheng,Li, Lan-bo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台