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Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples

文献类型： 外文期刊

作者： Li WeiHua 1 ; Meng Meng 2 ; He FangYang 4 ; Wan YuPing 4 ; Xue HuYin 2 ; Liu Wei 1 ; Yin WeiWei 1 ; Xu Jing 1 ; Feng CaiWei; 1 ;

作者机构： 1.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

2.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China

3.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China

4.Beijing Wanger Biotechnol Co Ltd, Beijing 102206, Peoples R China

5.Shandong Acad

关键词： diethylstilbestrol;ELISA;monoclonal antibody;drug residue

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2011 年 56 卷 8 期

页码：

收录情况： SCI

摘要： Based on the preparation of an anti-diethylstilbestrol (DES) monoclonal antibody, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ELISA) method for DES detection has been developed. The monoclonal antibody demonstrated high sensitivity to DES with an IC(50) value of 275 pg mL(-1) and detection limit (LOD) of 90 pg mL(-1). The specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related compounds of ethinyl estradiol (< 7%), estrone (< 0.1%), estriol (< 0.1%), and diethylstilbestrol benzoate (< 0.1%). Chicken, fish, shrimp, urine and bile spiked with different concentration of DES were detected by the developed method, and the recovery rates were greater than 79.5%. Intra- and inter-assay variations were about 6%. This method exhibited high stability with a coefficient of variation less than 10% in buffer and in real samples. The LODs in fish/shrimp, liver, feed and urine spiked with DES were 600, 600, 4800 and 600 pg mL(-1), respectively. These results confirmed that the antibody to DES was successfully produced and could be used to establish ELISA methods for DES detection in food producing animals.

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