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Genetic analysis of wild apple resources in Shandong province based on inter-simple sequence repeats (ISSR) and sequence-specific amplification polymorphism (S-SAP) markers

文献类型： 外文期刊

作者： He, Ping 1 ; Li, Linguang 1 ; Li, Huifeng 1 ; Wang, Haibo 1 ; Yang, Jianming 1 ; Wang, Yuxia 2 ;

作者机构： 1.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

2.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Liaoning, Peoples R China

关键词： Apple;wild resource;inter-simple sequence repeats (ISSR);sequence-specific amplification polymorphism (S-SAP)

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 46 期

页码：

收录情况： SCI

摘要： Apple (malus domestica Borkh.), which is a widely cultivated, important and economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Wild apple resources are important and they develop gradually in apple industry and genetic diversity. In this study, two molecular markers (inter-simple sequence repeats, ISSR and sequence-specific amplification polymorphism, S-SAP) were evaluated for genetic analysis of 31 wild apple resources in Shandong. A total of 20 ISSR primers were used and 110 polymorphic bands were amplified. Five S-SAP primer sets yielded a total of 496 bands, of which 201 (40.52%) were polymorphic. The similarity coefficient between resources ranged from 0.70 to 0.94 for ISSR analysis and from 0.66 to 0.95 using the S-SAP methodology. This study indicates that the results obtained based on the dendrograms constructed using unweighted pair-group using arithmetic average (UPGMA) cluster analysis were significantly correlated. The ISSR and S-SAP markers were found to be useful for wild apple resources identification and assessment of phonetic relationships.

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