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Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference

文献类型： 外文期刊

作者： Ma, Jin 1 ; Song, Yunzhi 1 ; Wu, Bin 1 ; Jiang, Mingsong 2 ; Li, Kaidong 1 ; Zhu, Changxiang 1 ; Wen, Fujiang 1 ;

作者机构： 1.Shandong Agr Univ, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Rice Sci Res Inst, Jining 272017, Peoples R China

关键词： Rice stripe virus;CP gene;SP gene;RNA interference;Transgenic rice

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2011 年 20 卷 6 期

页码：

收录情况： SCI

摘要： Rice stripe disease, with the pathogen Rice stripe virus (RSV), is one of the most widespread and severe virus diseases. Cultivating a resistant breed is an essential and efficient method in preventing rice stripe disease. Following RNA interference (RNAi) theory, we constructed three RNAi binary vectors based on coat protein (CP), special-disease protein (SP) and chimeric CP/SP gene sequence. Transgenic lines of rice cv. Yujing6 were generated through Agrobacterium-mediated transformation. We inoculated T1 generation plants from each line derived from CP/SP, CP, and SP transgenic rice plants with two RSV isolates from Shandong Province and Jiangsu Province using viruliferous vector insects. In these resistance assays, chimeric CP/SP RNAi lines showed stronger resistance against two isolates than CP or SP single RNAi lines. Stable integration and expression of RNAi transgenes were confirmed by Southern and northern blot analysis of independent transgenic lines. In the resistant transgenic lines, lower levels of transgene transcripts and specific short interference RNAs were observed relative to the susceptible transgenic plant, which showed that virus resistance was increased by RNAi. Genetic analysis demonstrated that transgene and virus resistance was stably inherited in the T2 progeny plants.

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