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Development of Ty1-copia retrotransposon-based S-SAP markers in strawberry (Fragaria x ananassa Duch.)

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文献类型：外文期刊

作者： He, Ping ¹ ; Ma, Yue ¹ ; Dai, Hongyan ¹ ; Li, Linguang ² ; Liu, Yuexue ¹ ; Li, He ¹ ; Zhao, Guiling ³ ; Zhang, Zhihong ¹ ;

作者机构： 1.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Liaoning, Peoples R China

2.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

3.Shenyang Agr Univ, Coll Forestry, Shenyang 110866, Liaoning, Peoples R China

关键词： FaRE1 retrotransposon;Strawberry;S-SAP;Polymorphism

期刊名称：SCIENTIA HORTICULTURAE （影响因子：3.463；五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2012 年 137 卷

页码：

收录情况： SCI

摘要： The most popular retrotransposon-based molecular marker system at the present time is the sequence-specific amplification polymorphism (S-SAP) system, which is widely used for various applications including phylogenetic analysis. In this study, we successfully developed a new S-SAP marker system based on FaRE1 retrotransposon in strawberry genome. Among 34 primer combinations used to develop the S-SAP marker system, seven primer combinations produced high quality and reliability banding patterns. A total of 288 polymorphic bands from 722 amplified bands were detected in 13 strawberry accessions, with a polymorphic ratio of 39.8%. Phylogenetic analysis showed that genetic coefficient ranged from 0.67 to 1.00 for 13 strawberry accessions. An 187 bp S-SAP band was detected from 50 strawberry plantlets regenerated from leaves which were cultured on MS media supplemented with 1.0 mg/l thidiazuron (TDZ) and 0.1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The available evidences proved S-SAP marker had high level of polymorphism in strawberry accessions. (C) 2012 Elsevier B.V. All rights reserved.

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Development of Ty1-copia retrotransposon-based S-SAP markers in strawberry (Fragaria x ananassa Duch.)

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意见箱

Development of Ty1-copia retrotransposon-based S-SAP markers in strawberry (Fragaria x ananassa Duch.)

作者其他论文 更多>>

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Full-length transcriptome and metabolism revealed the difference of callus formation of tea cutting under white, red and blue light

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意 见 箱

作者其他论文更多>>

意见箱