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Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells

文献类型： 外文期刊

作者： Wang Lin 1 ; Lin Shu Qian 2 ; He Yuan Long 2 ; Liu Gang 1 ; Wang Zhen Yong 1 ;

作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Inst Poultry Sci, Jinan 250023, Shandong, Peoples R China

关键词： Cadmium;Quercetin;Oxidative stress;Apoptosis;Proximal tubular cells;Primary cell culture

期刊名称：BIOMEDICAL AND ENVIRONMENTAL SCIENCES （ 影响因子：3.118； 五年影响因子：3.219 ）

ISSN： 0895-3988

年卷期： 2013 年 26 卷 4 期

页码：

收录情况： SCI

摘要： Objective To investigate the protective effects of quercetin on cadmium-induced cytotoxicity in primary cultures of rat proximal tubular (rPT) cells. Methods Primary cultures of rPT cells undergoing exponential growth were incubated with 1.0 mu g/mL quercetin and/or cadmium (2.5, 5.0 mu mol/L), in a serum-free medium at 37 degrees C at different time intervals. Commercial kits were used and flow cytometric analyses were performed on rPT cell cultures to assay apoptosis and oxidative stress. Results Exposure of rPT cells to cadmium acetate (2.5, 5.0 mu mol/L) induced a decrease in cell viability, caused an increase in apoptotic rate and apoptotic morphological changes. Simultaneously, elevation of intracellular reactive oxygen species, malondialdehyde and calcium levels, depletion of mitochondrial membrane potential and intracellular glutathione, and inhibition of Na+, K+-ATPase, Ca2+-ATPase, glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activities were revealed during the cadmium exposure of rPT cells. However, simultaneous supplementation with 1 mu g/mL quercetin protected rPT cells against cadmium-induced cytotoxicity through inhibiting apoptosis, attenuating lipid peroxidation, renewing mitochondrial function and elevating the intracellular antioxidants (non-enzymatic and enzymic) levels. Conclusion The present study has suggested that quercetin, as a widely distributed dietary antioxidant, contributes potentially to prevent cadmium-induced cytotoxicity in rPT cells.

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