您好，欢迎访问山东省农业科学院文献资源数据库平台

The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)

文献类型： 外文期刊

作者： Liu, Yu 1 ; Wang, Lu 1 ; Liu, Heng 1 ; Zhao, Rongrong 1 ; Liu, Bin 1 ; Fu, Quanjuan 2 ; Zhang, Yuanhu 1 ;

作者机构： 1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, 61 Dai Zong St, Tai An 271018, Shandong, Peoples R China

2.Shandong Inst Pomol, Tai An 271018, Shandong, Peoples R China

关键词： Tobacco;Senescence;Transcriptome;Reactive oxygen species (ROS)

期刊名称：BIOLOGICAL RESEARCH （ 影响因子：5.612； 五年影响因子：4.781 ）

ISSN： 0716-9760

年卷期： 2016 年 49 卷

页码：

收录情况： SCI

摘要： Background: alpha-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of alpha-farnesene synthase. The alpha-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winter pearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. Results: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O-2(center dot-)) had higher levels in transgenics compared to controls. Transgenic plants also had enhanced sensitivity to oxidative stress. The transcriptome of 8-week-old plants was studied to detect molecular changes. Differentially expressed unigene analysis showed that ubiquitin-mediated proteolysis, cell growth, and death unigenes were upregulated. Unigenes related to photosynthesis, antioxidant activity, and nitrogen metabolism were downregulated. Combined with the expression analysis of senescence marker genes, these results indicate that senescence started in the leaves of the transgenic plants at the vegetative growth stage. Conclusions: The antioxidative defense system was compromised and the accumulation of reactive oxygen species (ROS) played an important role in the premature aging of transgenic plants.

  • 相关文献

[1]Effects of selenium and sulfur on antioxidants and physiological parameters of garlic plants during senescence. Cheng Bo,Lian Hai-feng,Yu Xin-hui,Sun Ya-li,Sun Xiu-dong,Shi Qing-hua,Liu Shi-qi,Liu Ying-ying. 2016

[2]Regulation of endogenous hormones on post-harvest senescence in transgenic broccoli carrying an antisense or a sense BO-ACO 2 gene. Qin, Feifei,Wang, Cheng-rong,Wang, Ran,Ma, Gang,Qin, Feifei,Qin, Feifei,Xu, Hui-lian. 2009

[3]Effect of BO-ACO 2 gene on post-harvest senescence in transgenic broccoli (Brassica oleracea L. var. italica). Qin, Feifei,Wang, Chengrong,Wang, Ran,Ma, Gang,Qin, Feifei,Qin, Feifei,Xu, Hui-lian. 2011

[4]Signal transduction during wheat grain development. Kong, Lingan,Sun, Mingze,Guo, Honghai.

[5]Dead mycelium of Penicillium chrysogenum protects transplanted cotton plants agains fungal wilts in a saline field. Zhang, H. J.,Dong, H. Z.,Li, W. J.,Zhang, H. J.,Dong, H. Z.,Li, W. J..

[6]Mechanisms and regulation of senescence and maturity performance in cotton. Chen, Yizhen,Dong, Hezhong,Chen, Yizhen.

[7]Photosynthetic Characteristics of a Super High Yield Cultivar of Winter Wheat During Late Growth Period. Meng Qing-wei,Tian Ji-chun,Zhao Shi-jie,Sui Na,Li Meng. 2010

[8]Remobilization of vegetative nitrogen to developing grain in wheat (Triticum aestivum L.). Kong, Lingan,Xie, Yan,Hu, Ling,Feng, Bo,Li, Shengdong.

[9]Dry mycelium of Penicillium chrysogenum activates defense responses and restricts the spread of Tobacco Mosaic Virus in tobacco. Zhong, Yu,Chen, Zhuang-zhuang,Xie, Hong,Luo, Dan,Dai, Jin-ran,Wang, Jian-guang,Chen, Sui-yun,Peng, Jie-jun,Yan, Fei,Dong, He-zhong,Xie, Hong.

[10]Overexpression of Arabidopsis CBF1 gene in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. Yang, Jia-Sen,Meng, Jing-Jing,Bi, Yu-Ping,Xu, Ping-Li,Guo, Feng,He, Qi-Wei,Li, Xin-Guo,Yang, Jia-Sen,Meng, Jing-Jing,Bi, Yu-Ping,Xu, Ping-Li,Guo, Feng,Wan, Shu-Bo,Li, Xin-Guo,Wang, Ran,Wang, Ran.

[11]Transcriptome analysis of the roots at early and late seedling stages using Illumina paired-end sequencing and development of EST-SSR markers in radish. Wang, Shufen,He, Qiwei,Liu, Xianxian,Xu, Wenling,Li, Libin,Gao, Jianwei,Wang, Fengde,Wang, Xiufeng. 2012

[12]Transcriptome Analysis of Calcium and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development. Li, Yan,Meng, Jingjing,Yang, Sha,Guo, Feng,Zhang, Jialei,Geng, Yun,Cui, Li,Li, Xinguo,Wan, Shubo. 2017

[13]Transcriptome profiling of peanut gynophores revealed global reprogramming of gene expression during early pod development in darkness. Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Aiqin,Zhao, Shuzhen,Bi, Yuping,An, Jing,Wan, Shubo,Wang, Xingjun,Bi, Yuping,Wan, Shubo,Wang, Xingjun,Bi, Yuping,An, Jing,Zhao, Yanxiu,Wang, Xingjun. 2013

[14]Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution. Yang, Maohua,Mu, Tingzhen,Xing, Jianmin,Chen, Ruonan,Zhang, Xiang. 2017

[15]Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing. Wang, Fengde,Li, Libin,Li, Huayin,Liu, Lifeng,Zhang, Yihui,Gao, Jianwei,Wang, Xiaowu. 2012

[16]Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation. Lin, Haichao,Wang, Huaizhong,Wang, Yanping,Liu, Chang,Wang, Cheng,Guo, Jianfeng,Lin, Haichao,Wang, Yanping,Liu, Chang,Guo, Jianfeng. 2015

[17]Comparative Transcriptome Analysis to Reveal Genes Involved in Wheat Hybrid Necrosis. Cheng, Yan,Guo, Jiahui,Zheng, Xuelian,Deng, Kejun,Zhou, Jianping,Yang, Ennian,Liu, Cheng. 2014

[18]Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs. Wang, Yuding,Ma, Cai,Sun, Yi,Kang, Li,Jiang, Yunliang,Wang, Yuding,Li, Yi. 2017

[19]Molecular Link between Leaf Coloration and Gene Expression of Flavonoid and Carotenoid Biosynthesis in Camellia sinensis Cultivar 'Huangjinya'. Song, Lubin,Yao, Yuantao,Tao, Jihan,Ma, Qingping,Sun, Kang,Kaleri, Najeeb A.,Li, Xinghui,Zou, Zhongwei. 2017

[20]Differential gene expression and associated QTL mapping for cotton yield based on a cDNA-AFLP transcriptome map in an immortalized F-2. Liu, Renzhong,Wang, Baohua,Guo, Wangzhen,Zhang, Tianzhen,Liu, Renzhong,Wang, Liguo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台