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Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases

文献类型： 外文期刊

作者： Wu, Ribang 1 ; Chen, Leilei 2 ; Liu, Dan 1 ; Huang, Jiafeng 1 ; Zhang, Jiang 1 ; Xiao, Xiao 1 ; Lei, Ming 1 ; Chen, Yueli 1 ;

作者机构： 1.Cent S Univ, State Key Lab Med Genet, Sch Life Sci, Changsha 410013, Hunan, Peoples R China

2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Peoples R China

3.Shandong Acad Agr Sci, Shandong Prov Key Lab Agroprod Proc Technol, Jinan 250100, Peoples R China

关键词： bacterial extracellular proteases;antioxidant peptide;enzymatic hydrolysis;peptide purification;evaluation of antioxidant activity

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN： 1660-3397

年卷期： 2017 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Bacterial extracellular proteases from six strains of marine bacteria and seven strains of terrestrial bacteria were prepared through fermentation. Proteases were analyzed through substrate immersing zymography and used to hydrolyze the collagen and muscle proteins from a salmon skin byproduct, respectively. Collagen could be degraded much more easily than muscle protein, but it commonly showed weaker antioxidant capability. The hydrolysate of muscle proteins was prepared with crude enzymes from Pseudoalteromonas sp. SQN1 displayed the strongest activity of antioxidant in DPPH and hydroxyl radical scavenging assays (74.06% +/- 1.14% and 69.71% +/- 1.97%), but did not perform well in Fe2+ chelating assay. The antioxidant fractions were purified through ultrafiltration, cation exchange chromatography, and size exclusion chromatography gradually, and the final purified fraction U2-S2-I displayed strong activity of antioxidant in DPPH, hydroxyl radical scavenging assays (IC50 = 0.263 +/- 0.018 mg/mL and 0.512 +/- 0.055 mg/mL), and oxygen radical absorption capability assay (1.960 +/- 0.381 mmol.TE/g). The final purified fraction U2-S2-I possessed the capability to protect plasmid DNA against the damage of hydroxyl radical and its effect was similar to that of the original hydrolysis product. It indicated that U2-S2-I might be the major active fraction of the hydrolysate. This study proved that bacterial extracellular proteases could be utilized in hydrolysis of a salmon byproduct. Compared with collagen, muscle proteins was an ideal material used as an enzymatic substrate to prepare antioxidant peptides.

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