文献类型： 外文期刊
作者： Liu, Guoxia 1 ; Arnaud, Philippe 2 ; Offmann, Bernard 2 ; Picimbon, Jean-Francois 1 ;
作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
2.Univ Nantes, Prot Engn & Funct Unit, F-44322 Nantes, France
3.QILU Univ Technol, Sch Bioengn, Jinan 250353, Shandong, Peoples R China
关键词： biotype imprinting;lipometer;mutation sensor;RNA editing;genosensing
期刊名称：SENSORS （ 影响因子：3.576； 五年影响因子：3.735 ）
ISSN： 1424-8220
年卷期： 2017 年 17 卷 8 期
页码：
收录情况： SCI
摘要： Genotyping is the process of determining differences in the genetic make-up of an individual and comparing it to that of another individual. Focus on the family of chemosensory proteins (CSPs) in insects reveals differences at the genomic level across various strains and biotypes, but none at the level of individuals, which could be extremely useful in the biotyping of insect pest species necessary for the agricultural, medical and veterinary industries. Proposed methods of genotyping CSPs include not only restriction enzymatic cleavage and amplification of cleaved polymorphic sequences, but also detection of retroposons in some specific regions of the insect chromosome. Design of biosensors using CSPs addresses tissue-specific RNA mutations in a particular subtype of the protein, which could be used as a marker of specific physiological conditions. Additionally, we refer to the binding properties of CSP proteins tuned to lipids and xenobiotic insecticides for the development of a new generation of biosensor chips, monitoring lipid blood concentration and chemical environmental pollution.
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