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N6-methyladenosine mRNA methylation positively regulated the response of poplar to salt stress

文献类型： 外文期刊

作者： Zhao, Ye 1 ; Han, Kun-Jin 1 ; Tian, Yan-Ting 1 ; Jia, Kai-Hua 2 ; El-Kassaby, Yousry A. 3 ; Wu, Yue 1 ; Liu, Jie 1 ; Si, Hua-Yu 1 ; Sun, Yu-Han 1 ; Li, Yun 1 ;

作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, State Key Lab Tree Genet & Breeding,Engn Technol R, Beijing, Peoples R China

2.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Key Lab Crop Genet Improvement & Ecol & Physiol, Jinan, Shandong, Peoples R China

3.Univ British Columbia, Fac Forestry, Dept Forest & Conservat Sci, Vancouver, BC, Canada

4.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, State Key Lab Tree Genet & Breeding,Engn Technol R, Beijing 100083, Peoples R China

关键词： m(6)A methylation; methyltransferase; mRNA stability; salt stress

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.3； 五年影响因子：8.1 ）

ISSN： 0140-7791

年卷期： 2024 年 47 卷 5 期

页码：

收录情况： SCI

摘要： As the most abundant form of methylation modification in messenger RNA (mRNA), the distribution of N-6-methyladenosine (m(6)A) has been preliminarily revealed in herbaceous plants under salt stress, but its function and mechanism in woody plants were still unknown. Here, we showed that global m(6)A levels increased during poplar response to salt stress. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) revealed that m(6)A significantly enriched in the coding sequence region and 3 '-untranslated regions in poplar, by recognising the conserved motifs, AGACU, GGACA and UGUAG. A large number of differential m(6)A transcripts have been identified, and some have been proved involving in salt response and plant growth and development. Further combined analysis of MeRIP-seq and RNA-seq revealed that the m(6)A hypermethylated and enrich in the CDS region preferred to positively regulate expression abundance. Writer inhibitor, 3-deazaneplanocin A treatment increased the sensitivity of poplar to salt stress by reducing mRNA stability to regulate the expression of salt-responsive transcripts PagMYB48, PagGT2, PagNAC2, PagGPX8 and PagARF2. Furthermore, we verified that the methyltransferase PagFIP37 plays a positively role in the response of poplar to salt stress, overexpressed lines have stronger salt tolerance, while RNAi lines were more sensitive to salt, which relied on regulating mRNA stability in an m(6)A manner of salt-responsive transcripts PagMYB48, PagGT2, PagNAC2, PagGPX8 and PagARF2. Collectively, these results revealed the regulatory role of m(6)A methylation in poplar response to salt stress, and revealed the importance and mechanism of m(6)A methylation in the response of woody plants to salt stress for the first time.

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