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Comparative transcriptome analysis uncovers regulatory roles of long non-coding RNAs involved in resistance to powdery mildew in melon

文献类型： 外文期刊

作者： Gao, Chao 1 ; Sun, Jianlei 1 ; Dong, Yumei 1 ; Wang, Chongqi 1 ; Xiao, Shouhua 1 ; Mo, Longfei 2 ; Jiao, Zigao 1 ;

作者机构： 1.Shandong Acad Agr Sci, Vegetable Sci Observat & Expt Stn Huang Huai Dist, Shandong Key Lab Greenhouse Vegetable Biol,Shando, Minist Agr Shandong,Inst Vegetables & Flowers,Nat, Jinan 250100, Peoples R China

2.Jilin Agr Univ, Coll Hort, Changchun 130118, Peoples R China

关键词： Melon; Comparative transcriptome; Long non-coding RNA; Powdery mildew disease; Expression pattern

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs with more than 200 nucleotides in length, which play vital roles in a wide range of biological processes. Powdery mildew disease (PM) has become a major threat to the production of melon. To investigate the potential roles of lncRNAs in resisting to PM in melon, it is necessary to identify lncRNAs and uncover their molecular functions. In this study, we compared the lncRNAs between a resistant and a susceptible melon in response to PM infection. Results It is reported that 11,612 lncRNAs were discovered, which were distributed across all 12 melon chromosomes, and > 85% were from intergenic regions. The melon lncRNAs have shorter transcript lengths and fewer exon numbers than protein-coding genes. In addition, a total of 407 and 611 lncRNAs were found to be differentially expressed after PM infection in PM-susceptible and PM-resistant melons, respectively. Furthermore, 1232 putative targets of differently expressed lncRNAs (DELs) were discovered and gene ontology enrichment (GO) analysis showed that these target genes were mainly enriched in stress-related terms. Consequently, co-expression patterns between LNC_018800 and CmWRKY21, LNC_018062 and MELO3C015771 (glutathione reductase coding gene), LNC_014937 and CmMLO5 were confirmed by qRT-PCR. Moreover, we also identified 24 lncRNAs that act as microRNA (miRNA) precursors, 43 lncRNAs as potential targets of 22 miRNA families and 13 lncRNAs as endogenous target mimics (eTMs) for 11 miRNAs. Conclusion This study shows the first characterization of lncRNAs involved in PM resistance in melon and provides a starting point for further investigation into the functions and regulatory mechanisms of lncRNAs in the resistance to PM.

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[1]Comparative transcriptome analysis uncovers regulatory roles of long non-coding RNAs involved in resistance to powdery mildew in melon. Gao, Chao,Sun, Jianlei,Dong, Yumei,Wang, Chongqi,Xiao, Shouhua,Jiao, Zigao,Mo, Longfei. 2020

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