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Molecular Characterization of an Isoflavone 2 '-Hydroxylase Gene Revealed Positive Insights into Flavonoid Accumulation and Abiotic Stress Tolerance in Safflower

文献类型： 外文期刊

作者： Liu, Jianyu 1 ; Ahmad, Naveed 1 ; Hong, Yingqi 1 ; Zhu, Meihua 1 ; Zaman, Shah 3 ; Wang, Nan 1 ; Yao, Na 1 ; Liu, Xiuming 1 ;

作者机构： 1.Jilin Agr Univ, Coll Life Sci, Chinese Minist Educ Bioreactor & Pharmaceut Dev, Engn Res Ctr, Changchun 130118, Peoples R China

2.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China

3.Shandong Acad Agr Sci, Tea Res Inst, Jinan 250100, Peoples R China

关键词： CYP81E8; isoflavone 2 '-hydroxylase; constitutive expression; flavonoid biosynthesis; transgenic Arabidopsis; safflower

期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）

ISSN：

年卷期： 2022 年 27 卷 22 期

页码：

收录情况： SCI

摘要： Flavonoids with significant therapeutic properties play an essential role in plant growth, development, and adaptation to various environments. The biosynthetic pathway of flavonoids has long been studied in plants; however, its regulatory mechanism in safflower largely remains unclear. Here, we carried out comprehensive genome-wide identification and functional characterization of a putative cytochrome P45081E8 gene encoding an isoflavone 2'-hydroxylase from safflower. A total of 15 CtCYP81E genes were identified from the safflower genome. Phylogenetic classification and conserved topology of CtCYP81E gene structures, protein motifs, and cis-elements elucidated crucial insights into plant growth, development, and stress responses. The diverse expression pattern of CtCYP81E genes in four different flowering stages suggested important clues into the regulation of secondary metabolites. Similarly, the variable expression of CtCYP81E8 during multiple flowering stages further highlighted a strong relationship with metabolite accumulation. Furthermore, the orchestrated link between transcriptional regulation of CtCYP81E8 and flavonoid accumulation was further validated in the yellow- and red-type safflower. The spatiotemporal expression of CtCYP81E8 under methyl jasmonate, polyethylene glycol, light, and dark conditions further highlighted its likely significance in abiotic stress adaption. Moreover, the over-expressed transgenic Arabidopsis lines showed enhanced transcript abundance in OE-13 line with approximately eight-fold increased expression. The upregulation of AtCHS, AtF3'H, and AtDFR genes and the detection of several types of flavonoids in the OE-13 transgenic line also provides crucial insights into the potential role of CtCYP81E8 during flavonoid accumulation. Together, our findings shed light on the fundamental role of CtCYP81E8 encoding a putative isoflavone 2'-hydroxylase via constitutive expression during flavonoid biosynthesis.

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