文献类型： 外文期刊
作者： Xu, Xiang-Bo 1 ; Liu, Zhan-Xian 1 ; Zhang, Deng-Feng 1 ; Liu, Ying 1 ; Song, Wei-Bin 1 ; Li, Jian-Sheng 1 ; Dai, Jing-Rui 1 ;
作者机构： 1.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China
2.Shandong Acad Agr Sci, Inst Applicat Atom Energy, Jinan 250100, Peoples R China
关键词： Fertility restorer gene; Rf3; Pentatricopeptide repeat (PPR); Zea mays
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
ISSN： 0735-9640
年卷期： 2009 年 27 卷 4 期
页码：
收录情况： SCI
摘要： Using an in silico cloning approach, five putative maize pentatricopeptide repeat (PPR)-containing protein genes (PPR-814a, PPR-814b, PPR-814c, PPR-816, PPR-817) with complete open reading frames were identified in the inbred line S-Mo17(Rf3Rf3). The amino acid sequence indicated that these genes encoded mitochondrially targeted proteins containing repeats of a 35-aa PPR motif. The genes were mapped into the interval umc1525-bnlg1520 on chromosome 2. In a non-restoring genotype, we identified three homologous genes that contained deletions or nucleotide substitutions in the coding region. Sequence analysis revealed that one of the three genes (PPR-814a, PPR-814b, PPR-814c) could be considered a candidate restorer gene for S male sterility cytoplasm, and linkage analysis demonstrated that the genes co-segregated with the fertility restorer gene Rf3.
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