文献类型： 外文期刊

作者： Ma Zhuojun ¹ ; Bao Zhenmin ¹ ; Wang Sifeng ¹ ; Zhang Zhifeng ¹ ;

作者机构： 1.Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

3.Shandong Acad Sci, Inst Biol, Jinan 250014, Peoples R China

关键词： Urechis unicinctus;sulfide oxidation;mitochondria;ATP production;inhibitor

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2010 年 28 卷 3 期

页码：

收录情况： SCI

摘要： We measured sulfide-based ATP production by isolated mitochondria from four tissues of Urechis unicinctus and the effects of inhibitors of respiratory complexes on ATP production were evaluated. The results show that these mitochondria could oxidize sulfide to produce ATP. The yield of sulfide-stimulated ATP varied from 5 nmol ATP/min/mg to 90 nmol ATP/min/mg according to the sulfide concentration and the source of the mitochondria. The maximum ATP synthesis occurred in hindgut mitochondria using 5 mu mol/L sulfide as a substrate. The effects of inhibitors (Rotenone, Antimycin A, Cyanide, and Salicylhydroxamic acid) on mitochondrial ATP production varied with the source of the mitochondria. Our results indicate that sulfide-based ATP production and the associated electron transport pathway are tissue-specific in U. unicinctus.