您好，欢迎访问山东省农业科学院文献资源数据库平台

A novel naturally Phe206Tyr mutation confers tolerance to ALS-inhibiting herbicides in Alopecurus myosuroides

文献类型： 外文期刊

作者： Li, Jian 1 ; Li, Yanbang 2 ; Fang, Feng 1 ; Xue, Dexing 1 ; Li, Rumei 1 ; Gao, Xingxiang 1 ; Li, Mei 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Weed Sci Dept, 202 Gongye North Rd, Jinan 250100, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

关键词： Alopecurus myosuroides; Target -site resistance; Acetolactate synthase (ALS); Novel ALS resistance mutation; Imazethapyr

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）

ISSN： 0048-3575

年卷期： 2022 年 186 卷

页码：

收录情况： SCI

摘要： Herbicide-resistant weeds pose a serious threat to world food production. The rapid and widespread development of target-site based resistance limits the application of herbicides. Alopecurus myosuroides Huds. (blackgrass) has spread rapidly in winter wheat regions in China, and the field recommended dose of ALS herbicides no longer controls blackgrass populations in recent years. A highly resistant population TW18(R) was collected in 2018 from Shandong Province. Dose-response assays showed that the TW18 was resistant to mesosulfuron-methyl, flucarbazone-sodium, and imazethapyr, with resistance index values of 5.96, 6.1, and 4.09, respectively. DNA sequencing of the TW18 population revealed a Phe206Tyr (F206Y) mutation in the ALS, which was not yet reported. Blackgrass ALS gene with the F206Y mutation (R gene) was expressed in Arabidopsis and rice. Transgenic studies have shown that both Arabidopsis and rice expressing this R gene have resistance to imazethapyr. However, it did not confer resistance to tribenuron methyl and florasulam in transgenic Arabidopsis. This study showed that the F206Y substitution caused herbicide resistance in blackgrass. To our knowledge, this is the first-reported F206Y mutation of a weed species in the natural environment. Transgenic plants showed this functional site could be utilized to generate imazethapyr-resistant rice to control herbicide-resistant weed damage.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台