文献类型： 外文期刊
作者： Liu, Yaming 1 ; Cui, Yulin 1 ; Chen, Jun 1 ; Qin, Song 1 ; Chen, Gao 3 ;
作者机构： 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101418, Peoples R China
3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
4.Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
关键词： Synechocystis sp. PCC6803; Astaxanthin; beta-Carotene ketolase (bkt); Carotenoid hydroxylase (crtR-B); Metabolic engineering
期刊名称：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS （ 影响因子：4.401； 五年影响因子：5.278 ）
ISSN： 2211-9264
年卷期： 2019 年 44 卷
页码：
收录情况： SCI
摘要： Astaxanthin is a carotenoid with wide applications because of its strong antioxidant properties. It is mainly produced from the green alga Haematococcus pluvialis, but large-scale production remains a challenge. To provide another source, here, Synechocystis sp. PCC6803 was adopted as the chassis cell, and its carotenoid biosynthesis pathway was genetically extended to produce astaxanthin. The beta-carotene ketolase gene (bkt) and carotenoid hydroxylase gene (crtR-B) from H. pluvialis, which wild-type Synechocystis sp. PCC6803 lacks, were optimized based on the codon preference of Synechocystis sp. PCC6803 and heterologously expressed in that bacterium to create a strain capable of producing astaxanthin. The Synechocystis sp. PCC6803 mutant synthesized 4.81 +/- 0.06 mg astaxanthin per gram of cells (dry weight) after nitrogen starvation for 14 days. More than 30% of the total astaxanthin was in the free form, and 82.61% was the strong antioxidant 3S, 3'S-astaxanthin isoform. These results show the industrial application potential of metabolically-engineered Synechocystis sp. PCC6803 for astaxanthin production.
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