PbbHLH155 enhances iron deficiency tolerance in pear by directly activating PbFRO2 and PbbHLH38
文献类型： 外文期刊
作者： Dong, Weiyu 1 ; Liu, Lun 1 ; Sun, Yu 1 ; Xu, Xiaoqian 1 ; Guo, Guoling 1 ; Heng, Wei 1 ; Jiao, Huijun 2 ; Wei, Shuwei 2 ; Jia, Bing 1 ;
作者机构： 1.Anhui Agr Univ, Sch Hort, Hefei 230036, Peoples R China
2.Shandong Inst Pomol, Shandong Fresh Pear Cultivat & Breeding Engn Tech, Tai An 271000, Peoples R China
关键词： PbbHLH155; Fe deficiency; Pear calli; FCR activity; Acidification activity
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.1； 五年影响因子：6.2 ）
ISSN： 0981-9428
年卷期： 2024 年 213 卷
页码：
收录情况： SCI
摘要： Iron (Fe) deficiency is a general stress for many horticulture crops, causing leaf chlorosis and stunted growth. The basic-helix-loop-helix (bHLH) transcription factor (TF) was reported to function in Fe absorption; however, the regulatory mechanism of bHLH genes on iron absorption remains largely unclear in pear. In this study, we found that PbbHLH155 was significantly induced by Fe deficiency. Overexpression of PbbHLH155 in Arabidopsis thaliana and pear calli significantly increases resistance to Fe deficiency. The PbbHLH155-overexpressed Arabidopsis lines exhibited greener leaf color, higher Fe content, stronger Fe chelate reductase (FCR) and root acidification activity. The PbbHLH155 knockout pear calli showed lower Fe content and weaker FCR activity. Interestingly, PbbHLH155 inhibited the expressions of PbFRO2 and PbbHLH38, which were positive regulators in Fe-deficiency responses (FDR). Furthermore, yeast one-hybrid (Y1H) and Dual-Luciferase Reporter (DLR) assays revealed that PbbHLH155 directly binds to the promoters of PbFRO2 and PbbHLH38, thus activating their expression. Overall, our results showed that PbbHLH155 directly promote the expression of PbFRO2 and PbbHLH38 to activate FCR activity for iron absorption. This study provided valuable information for pear breeding.
- 相关文献
作者其他论文 更多>>
-
Chloroplast Genome Profiling and Phylogenetic Insights of the "Qixiadaxiangshui" Pear (Pyrus bretschneideri Rehd.1)
作者：Jiao, Huijun;Chen, Qiming;Wang, Hongwei;Ran, Kun;Dong, Ran;Dong, Xiaochang;Guan, Qiuzhu;Wei, Shuwei;Xiong, Chi;Wei, Shuwei
关键词："Qixiadaxiangshui" pear; chloroplast genome; comparative genome analysis; phylogenetic analysis
-
The pear genomics database (PGDB): a comprehensive multi-omics research platform for Pyrus spp.
作者：Chen, Shulin;Xu, Shaozhuo;Xue, Cheng;Zheng, Pengfei;Gu, Kaidi;Qiao, Zhiwen;Liu, Zhiying;Zhang, Mingyue;Sun, Manyi;Wu, Jun;Wei, Shuwei
关键词：Pear; Database; Genome; Transcriptome; Re-sequencing
-
Persulfidation of transcription factor MYB10 inhibits anthocyanin synthesis in red-skinned pear
作者：Yao, Gaifang;Gou, Shasha;Zhong, Tingying;An, Xin;Sun, Hongye;Sun, Chen;Hu, Kangdi;Zhang, Hua;Wei, Shuwei
关键词：
-
ETHYLENE RESPONSE FACTORS 4.1/4.2 with an EAR motif repress anthocyanin biosynthesis in red-skinned pears
作者：Sun, Hongye;Hu, Kangdi;Yao, Gaifang;Zhang, Hua;Wei, Shuwei
关键词：
-
The mechanism analysis of exogenous melatonin in limiting pear fruit aroma decrease under low temperature storage
作者：Wei, Shuwei;Jiao, Huijun;Wang, Hongwei;Ran, Kun;Dong, Ran;Dong, Xiaochang;Wang, Shaomin;Yan, Wenjing
关键词：Pear; Melatonin; Aroma
-
The regulation of plant lignin biosynthesis under boron deficiency conditions
作者：Dong, Xiaochang;Wei, Shuwei;Jiao, Huijun;Ran, Kun;Dong, Ran;Wang, Shaomin;Jiang, Cuncang
关键词：
-
Genome-wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus bretschneideri)
作者：Liu, Chunxin;Qiao, Xin;Li, Qionghou;Zeng, Weiwei;Wang, Xin;Chen, Yangyang;Wu, Xiao;Wu, Jun;Yin, Hao;Zhang, Shaoling;Wei, Shuwei
关键词：BAHD; Pear; Evolution; Rosaceae; Transcriptome; Volatile esters
