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The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four omega-3 AhFAD3 Members

文献类型： 外文期刊

作者： Peng, Zhenying 1 ; Ruan, Jian 1 ; Tian, Haiying 1 ; Shan, Lei 1 ; Meng, Jingjing 1 ; Guo, Feng 1 ; Zhang, Zhimeng 3 ; Ding, Hong 3 ; Wan, Shubo 2 ; Li, Xinguo 1 ;

作者机构： 1.Shandong Acad Agricuhural Sci, Shandong Prov Key Lab Genet Improvement Ecol & Ph, Biotech Res Ctr, Jinan 250100, Peoples R China

2.Shandong Normal Univ, Jinan 250100, Peoples R China

3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China

4.Shandong Acad Agr Sci, Jinan 250100, Peoples R China

关键词： Arachis hypogaea L; omega-3 fatty acid desaturase; Transcription profiling; Sub-cellular localization; Functional characterization; Salinity tolerance

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期： 2020 年 38 卷 2 期

页码：

收录情况： SCI

摘要： The synthesis of alpha-linolenic acid (ALA) requires the activity of omega-3 fatty acid desaturases (omega-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36 FAD genes, mapping to 16 of the species' 20 chromosomes. A phylogenetic analysis concluded that these genes belonged to six sub-families, namely stearoyl-acyl-acyl carrier protein desaturases (SAD), FAD2, FAD3, FAD4/5, FAD6 and FAD7/8. Of these, FAD3 and FAD7/8 encoded omega-3 FADs, while genes belonging to the other four sub-families encoded omega-6 FADs. Based on RNA-Seq data, each of the 36 FAD genes was shown to be transcribed in non-stressed plants, but there was variation between them with respect to which organs they were transcribed in. Four omega-3 AhFAD3 genes were functionally characterized; when expressed in Arabidopsis thaliana protoplasts, each was localized mainly in the endoplasmic reticulum, while within peanut, the genes were more strongly transcribed in the developing seed than in either the root or the leaf. When constitutively expressed in Arabidopsis thaliana, both the total fatty acid content of the seed and the relative contribution of ALA were increased. The transgenic seedlings also exhibited an improved level of survival when challenged by salinity stress.

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[1]The Family of Peanut Fatty Acid Desaturase Genes and a Functional Analysis of Four omega-3 AhFAD3 Members. Peng, Zhenying,Ruan, Jian,Tian, Haiying,Shan, Lei,Meng, Jingjing,Guo, Feng,Li, Xinguo,Peng, Zhenying,Shan, Lei,Wan, Shubo,Li, Xinguo,Zhang, Zhimeng,Ding, Hong,Wan, Shubo. 2020

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