Simultaneous detection of classical PRRSV, highly pathogenic PRRSV and NADC30-like PRRSV by TaqMan probe real-time PCR
文献类型： 外文期刊
作者： Qiu, Wenbin 1 ; Meng, Kai 1 ; Liu, Yanyan 1 ; Zhang, Yuyu 1 ; Wang, Zhao 4 ; Chen, Zhi 1 ; Yang, Jie 1 ; Sun, Wenbo 1 ; Guo, 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China
2.Shandong Normal Univ, Sch Life Sci, Jinan 250014, Peoples R China
3.Shandong Acad Agr Sci, Poultry Breeding Engn Technol Ctr Shandong Prov, Inst Poultry Sci, Shandong Key Lab Poultry Dis Diag & Immunol, Jinan 250023, Peoples R China
4.China Inst Vet Drug Control, 8 Nandajie, Beijing 100081, Peoples R China
关键词： Classical PRRSV; Highly pathogenic PRRSV; NADC30-like; PRRSV; TaqMan Probe real-time PCR
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2020 年 282 卷
页码：
收录情况： SCI
摘要： Porcine Reproductive and Respiratory Syndrome (PRRS), an acute infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is one of the most devastating diseases affecting the global swine industry. In order to establish a multiplex real-time PCR method for the simultaneous detection of the classical PRRSV (C-PRRSV) strain, the highly pathogenic PRRSV (HP-PRRSV) strain and NADC30-like PRRSV (NL-PRRSV) strain, we designed specific primers and TaqMan fluorescent probes based on the Nsp2 target gene sequence of these three different PRRSV strains, and designed American-type PRRSV (PRRSV-U) special primers and probes based on the relatively conserved target gene sequence of ORF7. The method established in this study can quickly and accurately detect and differentiate three types of strains of clinical tissue samples, respectively. This method plays a key role in the rapid diagnosis and determination of PRRSV.
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