文献类型： 外文期刊

作者： Xin, Ting ¹ ; Jia, Hong ¹ ; Ding, Jiabo ² ; Li, Pingjun ¹ ; Yang, Hongjun ³ ; Hou, Shaohua ¹ ; Yuan, Weifeng ¹ ; Guo, Xiaoyu ¹ ; Wang, Haichun ¹ ; Liang, Qianqian ¹ ; Li, Ming ¹ ; Wang, Bin ⁴ ; Zhu, Hongfei ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

2.China Inst Vet Drug Control, Beijing, Peoples R China

3.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan, Peoples R China

4.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

期刊名称：CLINICAL AND VACCINE IMMUNOLOGY （ 影响因子：3.233； 五年影响因子：2.562 ）

ISSN： 1556-6811

年卷期： 2013 年 20 卷 4 期

页码：

收录情况： SCI

摘要： Bovine tuberculosis (bTB) is a worldwide zoonosis caused mainly by Mycobacterium bovis. The traditional diagnostic method used often is the tuberculin skin test, which uses bovine purified protein derivatives (PPD-B). However, it is difficult to maintain uniformity of PPD-B from batch to batch, and it shares common antigens with nonpathogenic environmental mycobacteria. To overcome these problems, M. bovis-specific antigens that showed good T cell stimulation, such as CFP-10, ESAT-6, Rv3615c, etc., have been used in the skin test, but there have been no large-scale clinical studies on these antigens. In this study, two combinations (CFP-10/ESAT-6/TB10.4 protein cocktail and CFP-10/ESAT-6/Rv3872/MPT63 protein cocktail) were developed and used as stimuli in the skin test. Cattle were double-blind tested to assess the efficiency of the protein cocktail-based skin tests. The results showed that the CFP-10/ESAT-6/TB10.4 protein cocktail-based skin test can differentiate TB-infected cattle from Mycobacterium avium-infected ones and that it shows a high degree of agreement with the traditional tuberculin skin test (kappa = 0.8536) and gamma interferon (IFN-gamma) release assay (kappa = 0.8154). Compared to the tuberculin skin test, the relative sensitivity and relative specificity of the CFP-10/ESAT-6/TB10.4-based skin test were 87% and 97%, respectively., The relative sensitivity and relative specificity of the CFP-10/ESAT-6/TB10.4-based skin test were 93% and 92%, respectively, on comparison with the IFN-gamma release assay. The correlation between the increases in skin thickness observed after the inoculation of stimuli was high (PPD-B versus CFP-10/ESAT-6/TB10.4, Spearman r of 0.8435). The correlation between the optical density at 450 nm (OD450) obtained after blood stimulation with PPD-B and the increase in skin thickness observed after inoculation of the CFP-10/ESAT-6/TB10.4 protein cocktail was high (Spearman r = 0.7335). Therefore, the CFP-10/ESAT-6/TB10.4-based skin test responses correlate to traditional measures of bovine TB evaluation, including skin test and gamma interferon release assay.