A recombinant vaccine of Riemerella anatipestifer OmpA fused with duck IgY Fc and Schisandra chinensis polysaccharide adjuvant enhance protective immune response
文献类型： 外文期刊
作者： Yang, Shifa 1 ; Dong, Wenwen 1 ; Li, Guiming 1 ; Zhao, Zengcheng 1 ; Song, Minxun 1 ; Huang, Zhongli 1 ; Fu, Jian 1 ; Jia, Fengjuan 2 ; Lin, Shusqian 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Poultry Sci, Poultry Breeding Engn Technol Ctr Shandong Prov, Shandong Prov Key Lab Poultry Dis Diag & Immunol, Jinan 250023, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc,Minist Agr, Jinan 250100, Shandong, Peoples R China
关键词： R. anatipestifer; IgY Fc; P. pastoris expression; Subunit vaccine; SCP; Duck peritoneal macrophages
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 136 卷
页码：
收录情况： SCI
摘要： Riemerella anatipestifer (R. anatipestifer) causes septicemia and infectious serositis in domestic ducks, leading to high mortality and great economic losses worldwide. Vaccination is currently considered the best strategy to prevent R. anatipestifer infection in ducklings. In this study, we fused the duck IgY Fc gene to the outer membrane protein A (ompA) of R. anatipestifer. The eukaryotic expression plasmid carrying the fusion gene was transformed into Pichia pastoris (P. pastoris) to express the recombinant ompA and ompA-Fc proteins. Then, the effects of fused Fc on the vitality and antigen processing efficiency of duck peritoneal macrophages (PMo) were evaluated in vitro, whereas their immunogenicity was evaluated in vivo. Furthermore, Schisandra chinensis polysaccharide (SCP) was used to evaluate its immune-conditioning effects on the activation of PMo. SCP was also used as adjuvant to investigate immunomodulation on immunoresponses induced by the fused ompA-Fc in ducklings. The conventional Freund's incomplete adjuvant served as the control of SCP. Notably, ompA-Fc promoted phagocytosis of PMo and significantly increased serum antibody titers, CD4(+ )and CD8(+) T-lymphocyte counts, lymphocyte transformation rate, and serum levels of IL-2 and IL-4. In addition, ducklings injected with the ompA-Fc vaccine exhibited considerably greater resistance to the R. anatipestifer challenge than those that received vaccines based on standalone ompA. Of note, SCP was demonstrated to boost the secretion of nitric oxide (NO), IL-1 beta, IL-6, TNF-alpha, and IFN-beta by duck macrophages. In addition, the supplementation of SCP adjuvant to the ompA-Fc vaccines led to the further enhancement of immune response and vaccine protection. The dose of 200 mu g/mL showed the most pronounced effects. This study provided valuable insights into protective strategies against R. anatipestifer infection.
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