Novel Antioxidant Peptides Identified from Arthrospira platensis Hydrolysates Prepared by a Marine Bacterium Pseudoalteromonas sp. JS4-1 Extracellular Protease
文献类型: 外文期刊
作者: Liu, Congling 1 ; Chen, Gong 1 ; Rao, Hailian 1 ; Xiao, Xun 1 ; Chen, Yidan 1 ; Wu, Cuiling 2 ; Bian, Fei 3 ; He, Hailun 1 ;
作者机构: 1.Cent South Univ, Sch Life Sci, State Key Lab Med Genet, Changsha 410013, Peoples R China
2.Changzhi Med Coll, Dept Biochem, Changzhi 046000, Peoples R China
3.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Jinan 250100, Peoples R China
关键词: phycobiliprotein; antioxidant peptide; bacterial extracellular protease; enzymatic hydrolyzation; C; elegans
期刊名称:MARINE DRUGS ( 影响因子:5.4; 五年影响因子:5.5 )
ISSN:
年卷期: 2023 年 21 卷 2 期
页码:
收录情况: SCI
摘要: Crude enzymes produced by a marine bacterium Pseudoalteromonas sp. JS4-1 were used to hydrolyze phycobiliprotein. Enzymatic productions showed good performance on DPPH radical and hydroxyl radical scavenging activities (45.14 +/- 0.43% and 65.11 +/- 2.64%, respectively), especially small peptides with MWCO <3 kDa. Small peptides were fractioned to four fractions using size-exclusion chromatography and the second fraction (F2) had the highest activity in hydroxyl radical scavenging ability (62.61 +/- 5.80%). The fraction F1 and F2 both exhibited good antioxidant activities in oxidative stress models in HUVECs and HaCaT cells. Among them, F2 could upregulate the activities of SOD and GSH-Px and reduce the lipid peroxidation degree to scavenge the ROS to protect Caenorhabditis elegans under adversity. Then, 25 peptides total were identified from F2 by LC-MS/MS, and the peptide with the new sequence of INSSDVQGKY as the most significant component was synthetized and the ORAC assay and cellular ROS scavenging assay both illustrated its excellent antioxidant property.
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