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Molecular Cloning and Functional Characterization of FOUR LIPS in Pomegranate, a Protein Involved in Regulating the Gravitropic Set-Point Angle of Adventitious Roots

文献类型： 外文期刊

作者： Wang, Zenghui 1 ; Li, Jialin 2 ; Wang, Chuanzeng 1 ; Feng, Lijuan 1 ; Yin, Yanlei 1 ;

作者机构： 1.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

2.Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Peoples R China

关键词： Auxin; FOUR LIPS; Gravitropic set-point angle; PIN; Self-rooted pomegranate seedling

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：3.15； 五年影响因子：2.659 ）

ISSN： 1226-9239

年卷期：

页码：

收录情况： SCI

摘要： Self-rooted pomegranate seedlings are widely used in the horticultural industry to cut costs and time. However, these seedlings produce shallow roots that exhibit poor cold resistance. Thus, deeper adventitious roots generated through gravitropism are imperative for seedling survival, and understanding the molecular mechanisms of gravitropism can facilitate improved breeding techniques. We hypothesized that gravitropism in pomegranate is partially controlled by pomegranate FOUR LIPS (PgFLP), an R2R3-MYB protein that interacts with and controls the transcriptional expression of PgPIN10, which facilitates transmembrane auxin signaling. We studied subcellular localization of PgFLP, quantified auxin levels, and measured gravitropic set-point angle (GSA) to investigate the underlying mechanisms regulating PgPIN10 expression during the formation of GSA in pomegranate adventitious roots. We found that PgFLP was localized to the nucleus based on use of green florescent proteins, suggesting that this protein is a transcription factor. When using the tractable 35S::PgFLP, we observed stronger gravitational response in overexpression lines leading to a narrower GSA than in the wild-type Arabidopsis, and the expression of PgFLP and PgPIN10 in 'Lanbaoshi' (LBS; a deep-rooted cultivar) was higher than that in 'Taishanhong' (TSH; a shallow-rooted cultivar), which indicates that PgFLP may participate in regulating the GSA of adventitious roots via PgPIN10 in pomegranate. Taken together, our results indicate that the pomegranate R2R3-MYB transcription factor, PgFLP, plays a vital role in setting the GSA of adventitious roots in this crop species.

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