The effect of the SNP g.18475 A > G in the 3'UTR of NCF4 on mastitis susceptibility in dairy cattle
文献类型： 外文期刊
作者： Ju, Zhihua 1 ; Wang, Changfa 1 ; Wang, Xiuge 1 ; Yang, Chunhong 1 ; Zhang, Yan 1 ; Sun, Yan 1 ; Jiang, Qiang 1 ; Li, Rongling 1 ; Li, Jianbin 1 ; Zhong, Jifeng 2 ; Huang, Jinming;
作者机构： 1.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, 159 North Ind Rd, Jinan 250131, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, 159 North Ind Rd, Jinan 250131, Shandong, P
关键词： Bovine; NCF4; Mastitis; SCS; Bta-miR-2426; 3'UTR SNP
期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）
ISSN： 1355-8145
年卷期： 2018 年 23 卷 3 期
页码：
收录情况： SCI
摘要： Neutrophil cytosolic factor 4 (NCF4) is a member of the nicotinamide adenine dinucleotide phosphate oxidase subunit. This protein functions as an essential factor in the host defense against the progression of bacterial infection. To explore the variability of the NCF4 gene and the susceptibility of cows to mastitis, NCF4 functional single nucleotide polymorphism (SNP) of the 3' untranslated region (3'UTR) and its targeted microRNA (miRNA) were identified. One SNP g.18475 A > G in the 3'UTR of NCF4 was found within the binding seed region of bta-miR-2426. We constructed two recombinant pMIR-REPORT (TM) vectors with the A or G allele in the g.18475 locus and transiently co-transfected the vectors in human embryo kidney 293T (HEK 293T) cells, along with bta-miR-2426 mimics. A luciferase assay indicated that this SNP affects the binding of NCF4 and bta-miR-2426. In addition, the association analysis results showed that cows with the GG genotype in SNP g.18475 A > G had a relatively lower SCS value than cows with the AA genotype. Finally, quantitative real-time PCR (RT-qPCR) results showed that the cows with genotype GG had a relatively higher expression of NCF4 mRNA compared to the cows with genotype AA. NCF4 expression was regulated by the miRNA-mRNA interaction mechanism, and an important role for NCF4 in mastitis susceptibility in dairy cow was suggested.
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