您好,欢迎访问山东省农业科学院文献资源数据库平台

microRNA/microRNA* complementarity is important for the regulation pattern of NFYA5 by miR169 under dehydration shock in Arabidopsis

文献类型: 外文期刊

作者: Du, Qingguo 1 ; Zhao, Meng 2 ; Gao, Wei 1 ; Sun, Suzhen 1 ; Li, Wen-Xue 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.Shandong Acad Agr Sci, Maize Inst, Natl Engn Lab Wheat & Maize, Jinan 250100, Peoples R China

关键词: Arabidopsis;microRNA;microRNA* complementarity;miR169;regulation pattern;dehydration

期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )

ISSN: 0960-7412

年卷期: 2017 年 91 卷 1 期

页码:

收录情况: SCI

摘要: MicroRNAs regulate gene expression at the mRNA and translational levels. Although our previous research showed that expression of miR169 and one of its targets, NFYA5, is down- and up-regulated by drought stress, respectively, the current study shows that expression of both miR169 and NFYA5 are induced by dehydration shock. Unlike overexpression of MIR169a/b, overexpression of MIR169i/l did not decrease NFYA5 transcripts but increased NFYA5 protein levels. NFYA5 protein abundance also increased in mir169a knock-out mutants and 35S::MIR169l mir169a double mutants. When bulge #11 and bulge #16 in the miR169/miR169* duplex were mutated, both NFYA5 transcripts and the corresponding protein levels were lower in 35S::MIR169l*-mut transgenic plants than in wild-type (WT) plants, and the 35S::MIR169l*-mut transgenic plants were as sensitive to drought stress as the 35S::MIR169a plants. The mRNA and protein levels of NFYA5 did not differ substantially between WT and 35S::MIR169a*-mut transgenic plants when the two bulges were introduced in the miR169a/miR169a* duplex. Both bulge #11 and bulge #16 in the miR169/miR169* duplex were essential for different regulation patterns of NFYA5 by miR169a and miR169l. These results increase the understanding of regulatory specialization in one MIR family, and also increase our understanding of the importance of microRNA/microRNA* secondary structure.

  • 相关文献

[1]中国荷斯坦牛HSF1基因microRNA SNPs与耐热性能的相关性研究. 李秋玲,鞠志花,贾祥捷,黄金明,李建斌,李荣岭,李芳,王长法,仲跻峰. 2011

[2]奶牛OLR1 3'UTR A223C多态的生物学信息分析. 张传生,耿立英,尹春光,曹顶国,刘铮铸,付志新. 2011

[3]4条小麦保守MicroRNAs的表达谱分析及其靶基因预测. 闫妍,韩冉,赵惠贤. 2012

[4]miRBase-microRNA序列数据库. 高青,鞠志花,王长法,李国荣. 2011

[5]鸡microRNA基因“seed”区4个SNPs的群体遗传分布. 耿立英,张传生,尹春光,曹顶国,付志新. 2011

[6]奶牛OLR1 3′UTR A223C多态的生物学信息分析(英文). 张传生,耿立英,尹春光,曹顶国,刘铮铸,付志新. 2010

[7]High-throughput sequencing discovery of conserved and novel microRNAs in Chinese cabbage (Brassica rapa L. ssp pekinensis). Wang, Fengde,Li, Libin,Liu, Lifeng,Li, Huayin,Zhang, Yihui,Gao, Jianwei,Yao, Yingyin,Ni, Zhongfu. 2012

[8]Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice. Sun, Wei,wu, Xiu,Xie, Xianzhi,Xu, Xiao Hui,Lu, Xingbo,Sun, Hongwei,Wang, Yong. 2015

[9]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[10]Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein Cattle. Huang, Jinming,Ju, Zhihua,Li, Qiuling,Hou, Qinlei,Wang, Changfa,Li, Jianbin,Li, Rongling,Wang, Lingling,Sun, Tao,Hang, Suqin,Gao, Yundong,Hou, Minghai,Zhong, Jifeng.

[11]Virus-Based MicroRNA Silencing and Overexpressing in Common Wheat (Triticum aestivum L.). Jian, Chao,Chi, Qing,Wang, Shijuan,Ma, Meng,Liu, Xiangli,Zhao, Huixian,Han, Ran,Zhao, Huixian. 2017

[12]A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs. Bai, Ying,Huang, Jin-Ming,Liu, Gang,Zhang, Ji-Bin,Liu, Cheng-Kun,Fang, Mei-Ying,Huang, Jin-Ming,Wang, Jian-Ying. 2014

[13]MicroRNA expression analysis of rosette and folding leaves in Chinese cabbage using high-throughput Solexa sequencing. Wang, Fengde,Li, Huayin,Zhang, Yihui,Li, Jingjuan,Li, Libin,Liu, Lifeng,Wang, Lihua,Wang, Cuihua,Gao, Jianwei.

[14]Response of microRNAs to cold treatment in the young spikes of common wheat. Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying,Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying,Song, Guoqi,Zhang, Rongzhi,Zhang, Shujuan,Li, Yulian,Gao, Jie,Han, Xiaodong,Chen, Mingli,Wang, Jiao,Li, Wei,Li, Genying. 2017

[15]Mining, identification and function analysis of microRNAs and target genes in peanut (Arachis hypogaea L.). Zhang, Tingting,Hu, Shuhao,Yan, Caixia,Li, Chunjuan,Zhao, Xiaobo,Shan, Shihua,Hu, Shuhao,Wan, Shubo.

[16]ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis. Yu, Yanli,Li, Yanjiao,Zhao, Meng,Li, Wencai,Sun, Qi,Li, Wenlan,Meng, Zhaodong,Jia, Fengjuan,Jia, Fengjuan,Li, Nana. 2017

[17]The genetics of phytate and phosphate accumulation in seeds and leaves of Arabidopsis thaliana, using natural variation. Bentsink, L,Yuan, K,Koornneef, M,Vreugdenhil, D. 2003

[18]Homologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis. Li, Lingli,Wei, Jing,Huang, Guixue,Zhang, Dun,Liu, Yong,Zhang, Lingyun,Yu, Yanli.

作者其他论文 更多>>