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Isolation and genomic characterization of a novel Autographiviridae bacteriophage IME184 with lytic activity against Klebsiella pneumoniae

文献类型： 外文期刊

作者： Li, Fei 1 ; Tian, Fengjuan 1 ; Nazir, Amina 4 ; Sui, Shujing 3 ; Li, Mengzhe 1 ; Cheng, Dongxiao 1 ; Nong, Siqin 1 ; Ali, Azam 5 ; Kakar, Mohib-Ullah 6 ; Li, Lu 7 ; Feng, Qiang 2 ; Tong, Yigang 1 ;

作者机构： 1.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 10029, Peoples R China

2.Qingdao Univ, Ctr Clin Lab, Affiliated Taian City Cent Hosp, Tai An 271000, Shandong, Peoples R China

3.Qingdao Univ, Dept Gastroenterol, Affiliated Taian City Cent Hosp, Tai An 271000, Shandong, Peoples R China

4.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan Ind North Rd 202, Jinan, Shandong, Peoples R China

5.Univ Lahore, Inst Mol Biol & Biotechnol IMBB, Lahore, Pakistan

6.Lasbela Univ Agr Water & Marine Sci LUAWMS, Fac Marine Sci, Uthal 90150, Balochistan, Pakistan

7.Taian Ctr Dis Control & Prevent, Phys & Chem Lab, Tai An 271000, Shandong, Peoples R China

关键词： Phage; Multi-drug resistance; Klebsiella pneumoniae; Autographiviridae; Nosocomial infections

期刊名称：VIRUS RESEARCH （ 影响因子：5.0； 五年影响因子：4.0 ）

ISSN： 0168-1702

年卷期： 2022 年 319 卷

页码：

收录情况： SCI

摘要： Klebsiella pneumoniae, a multidrug resistant bacterium that causes nosocomial infections including septicemia, pneumonia etc. Bacteriophages are potential antimicrobial agents for the treatment of antibiotic resistant bacteria. In this study, a novel bacteriophage IME184, was isolated from hospital sewage against clinical multi-drug resistant Klebsiella pneumoniae. Transmission electron microscopy and genomic characterization exhibited this phage belongs to the Molineuxvirinae genus, Autographiviridae family. Phage IME184 possessed a double-stranded DNA genome composed of 44,598 bp with a GC content of 50.3%. The phage genome encodes 57 open reading frames, out of 26 are hypothetical proteins while 31 had assigned putative functions. No tRNA, virulence related or antibiotic resistance genes were found in phage genome. Comparative genomic analysis showed that phage IME184 has 94% similarity with genomic sequence of Klebsiella phage K1-ULIP33 (MK380014.1). Multiplicity of infection, one step growth curve and host range of phage were also measured. According to findings, Phage IME184 is a promising biological agent that infects Klebsiella pneumoniae and can be used in future phage therapies.

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