文献类型： 外文期刊

作者： Yu, X. M. ¹ ; Yu, T. ¹ ; Yin, G. H. ³ ; Dong, Q. L. ¹ ; An, M. ¹ ; Wang, H. R. ¹ ; Ai, C. X. ¹ ;

作者机构： 1.Shandong Inst Pomol, Tai An, Shandong, Peoples R China

2.Shandong Agr Univ, Tai An, Shandong, Peoples R China

3.Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA

4.Chinese Acad Agr Sci, Res Inst Pomol, Xincheng, Liaoning, Peoples R China

关键词： Bacillus subtilis;BIOLOG ECO microplate analysis;Functional diversity;Glyphosate degradation;Microbial communities;Soil bioremediation

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Glyphosate and glyphosate-containing herbicides have an adverse effect on mammals, humans, and soil microbial ecosystems. Therefore, it is important to develop methods for enhancing glyphosate degradation in soil through bioremediation. We investigated the potential of glyphosate degradation and bioremediation in soil by Bacillus subtilis Bs-15. Bs-15 grew well at high concentrations of glyphosate; the maximum concentration tolerated by Bs-15 reached 40,000 mg/L. The optimal conditions for bacterial growth and glyphosate degradation were less than 10,000 mg/L glyphosate, with a temperature of 35 degrees C and a pH of 8.0. Optimal fermentation occurred at 180 rpm for 60 h with an inoculum ratio of 4%. Bs-15 degraded 17.65% (12 h) to 66.97% (96 h) of glyphosate in sterile soil and 19.01% (12 h) to 71.57% (96 h) in unsterilized soil. Using a BIOLOG ECO plate test, we observed no significant difference in average well color development values between the soil inoculated with Bs-15 and the control soil before 72 h, although there was a significant difference (P < 0.01) after 72 h. In the presence of Bs-15, the 5 functional diversity indices (Shannon index, Shannon uniformity, Simpson index, McIntosh index, and McIntosh uniformity) were greater (P < 0.01) compared with the control soil. These results indicate that Bs-15 could be used to alleviate contamination from glyphosate-containing herbicides, increasing the microbial functional diversity in glyphosate-contaminated soils and thus enhancing the bioremediation of glyphosate-contaminated soils.