文献类型： 外文期刊

作者： Wang, Jing-Shan ¹ ; Sui, Jiong-Ming ¹ ; Xie, Yong-Dun ² ; Guo, Hui-Jun ² ; Qiao, Li-Xian ¹ ; Zhao, Li-Lan ³ ; Yu, Shan ¹ ;

作者机构： 1.Qingdao Agr Univ, Coll Life Sci, Key Lab Plant Biotechnol Univ Shandong Prov, Qingdao 266109, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Natl Ctr Space Mutagenesis Crop Improvement, Beijing 100081, Peoples R China

3.Shandong Agr Broadcasting & Televis Sch, Penglai 265600, Peoples R China

4.Shandong Acad Agr Sci, Peanut Res Inst, Qingdao 266100, Peoples R China

关键词： peanut (Arachis hypogaea L.);fast neutron irradiation;mutagenesis;in vitro culture;mutant

期刊名称：JOURNAL OF RADIATION RESEARCH （ 影响因子：2.724； 五年影响因子：2.587 ）

ISSN： 0449-3060

年卷期： 2015 年 56 卷 3 期

页码：

收录情况： SCI

摘要： Induced mutations have played an important role in the development of new plant varieties. In this study, we investigated the effects of fast neutron irradiation on somatic embryogenesis combined with plant regeneration in embryonic leaflet culture to develop new peanut (Arachis hypogaea L.) germplasm for breeding. The dry seeds of the elite cultivar Luhua 11 were irradiated with fast neutrons at dosages of 9.7, 14.0 and 18.0 Gy. The embryonic leaflets were separated and incubated in a medium with 10.0-mg/l 2,4-D to induce somatic embryogenesis. Next, they were incubated in a medium with 4.0-mg/l BAP for plant regeneration. As the irradiation dosage increased, the frequency of both somatic embryo formation and plantlet regeneration decreased. The regenerated plantlets were grafted onto rootstocks and were transplanted into the field. Later, the mature seeds of the regenerated plants were harvested. The M-2 generation plants from most of the regenerated cultivars exhibited variations and segregation in vigor, plant height, branch and pod number, pod size, and pod shape. To determine whether the phenotypes were associated with genomic modification, we compared the DNA polymorphisms between the wild-type plants and 19 M-3-generation individuals from different regenerated plants. We used 20 pairs of simple sequence repeat (SSR) primers and detected polymorphisms between most of the mutants and the wild-type plants (Luhua 11). Our results indicate that using a combination of fast neutron irradiation and tissue culture is an effective approach for creating new peanut germplasm.