Comparative proteomic analysis provides insight into 10-hydroxy-2-decenoic acid biosynthesis in honey bee workers
文献类型： 外文期刊
作者： Yang, Xiao-Hui 1 ; Yang, Shi-Fa 2 ; Wang, Rui-Ming 1 ;
作者机构： 1.Qilu Univ Technol, Key Lab Shandong Microbial Engn, Jinan 250353, Peoples R China
2.Shandong Acad Agr Sci, Jinan 250023, Peoples R China
关键词： Honey bee;10-Hydroxy-2-decenoic acid;Biosynthesis;Proteomics;RNAi
期刊名称：AMINO ACIDS （ 影响因子：3.52； 五年影响因子：3.6 ）
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收录情况： SCI
摘要： 10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis was performed in the MGs of workers with different 10-HDA production. In total, 59 up-regulated protein species representing 45 unique proteins were identified in high 10-HDA-producing workers by 2-DE-MALDI-TOF/TOF MS. These proteins were involved in carbohydrate/energy metabolism, fatty acid metabolism, protein metabolism and folding, antioxidation, cytoskeleton, development and cell signaling. Proteins related to fatty acid metabolism, including fatty acid synthase and beta-oxidation enzymes, are potentially crucial proteins involved in 10-HDA biosynthesis pathway. And RNA interference (RNAi) results demonstrated that knockdown of electron transfer flavoprotein subunit beta (ETF-beta), one of the protein related to fatty acid metabolism, decreased 10-HDA production of worker bees, suggesting that ETF-beta was necessary for 10-HDA biosynthesis. This study reveals the characteristics of MGs of worker bees at different developmental stages and proteins associated with 10-HDA biosynthesis, which provides the first insight into the molecular mechanism of 10-HDA biosynthesis.
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