您好，欢迎访问山东省农业科学院文献资源数据库平台

Cloning and Expression Analysis of Eight Upland Cotton Pentatricopeptide Repeat Family Genes

文献类型： 外文期刊

作者： Han, Zongfu 1 ; Qin, Yuxiang 2 ; Kong, Fanjin 1 ; Deng, Yongsheng 1 ; Wang, Zongwen 1 ; Shen, Guifang 1 ; Wang, Jinghu 1 ;

作者机构： 1.Shandong Acad Agr Sci, Cotton Res Ctr, Jinan 250100, Peoples R China

2.Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Peoples R China

关键词： Cotton;PPR protein;Cloning;Expression;Salt tolerance

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The pentatricopeptide repeat (PPR) gene family is one of the largest gene families in plants. Most PPR genes are localized in mitochondria and chloroplasts functioning in regulation of plant growth and development, fertility restoration for cytoplasmic male sterility (CMS), and stress defense. In this study, using in silico cloning and PCR amplification with degenerate primers based on Arabidopsis PPR genes, we cloned eight new full-length PPR genes encoding protein sequences ranging from 458 to 875 amino acids, with 8 to 16 repetitive PPR elements in upland cotton and all of them lack introns. Expression analysis revealed that eight PPR genes were differently expressed in roots, stems, leaves, and floral buds. As for GhI12, its expression in floral buds at days 3-5 was significantly higher in line 777R (restorer line) than in line 777A (CMS line). Further tests with real-time PCR showed that GhI12 expression peaked at day 3 in 777R, followed by a gradual decline, while its expression fluctuated in 777A, peaking at day 5 and day 13. In addition, Gh155c17 and GhI12 were upregulated under salt stress. This is the first report of upland cotton PPR genes involved in salt stress response.

  • 相关文献

[1]IMPROVED NUTRIENT UPTAKE ENHANCES COTTON GROWTH AND SALINITY TOLERANCE IN SALINE MEDIA. Dai, J. L.,Duan, L. S.,Dong, H. Z.,Dai, J. L.. 2014

[2]Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Pan, Hongyu,Wei, Yi,Xin, Furong,Zhou, Mingguo,Zhang, Shihong.

[3]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

[4]Overexpression of an S-like ribonuclease gene, OsRNS4, confers enhanced tolerance to high salinity and hyposensitivity to phytochrome-mediated light signals in rice. Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Li, Yaping,Liu, Qianqian,Xie, Xianzhi,Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Xie, Xianzhi. 2014

[5]Isolation of Arachis hypogaea Na+/H+ antiporter and its expression analysis under salt stress. Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo,Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo,Xing, Jinyi,Wang, Baozhi,Jia, Kunhang,Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo. 2011

[6]Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxide-associated 1 mutant Atnoa1. Qiao, Weihua,Yu, Liang,Fan, Liu-Min,Xiao, Shouhua.

[7]Isolation and functional characterisation of CDPKs gene from Arachis hypogaea under salt stress. Li, Yan,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Li, Yan,Wan, Shu-Bo,Fang, Feng,Xia, Guang-Min.

[8]Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco. Li, Tian,Sun, Jingkuan,Bi, Yuping,Peng, Zhenying.

[9]Identification of QTLs for salt tolerance at germination and seedling stage of Sorghum bicolor L. Moench. Wang, Hailian,Chen, Guiling,Zhang, Huawen,Liu, Bin,Yang, Yanbing,Qin, Ling,Chen, Erying,Guan, Yanan.

[10]Specific Expression of Maize SBEIIb Promoter Mediated by Different Promoter Region in Transgenic Tobacco Plants. Sun Cui-xia,Li Meng,Wang Xiao-peng,Zhang Guo-dong,Tian Yan-chen,Wang Ze-li,Han Jing. 2009

[11]Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae). Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng. 2014

[12]A simplified pruning method for profitable cotton production in the Yellow River valley of China. Dai, Jianlong,Luo, Zhen,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Lu, Hequan,Li, Zhenhuai,Xin, Chengsong,Kong, Xiangqiang,Eneji, A. Egrinya,Dong, Hezhong.

[13]Dry mycelium of Penicillium chrysogenum induces expression of pathogenesis-related protein genes and resistance against wilt diseases in Bt transgenic cotton. Chen, Suiyun,Dong, Hezhong,Fan, Yuqin,Li, Weijiang,Cohen, Yigal. 2006

[14]Effects of Soil Salinity and Plant Density on Yield and Leaf Senescence of Field-Grown Cotton. Zhang, H. J.,Dong, H. Z.,Li, W. J.,Zhang, D. M.,Zhang, H. J.. 2012

[15]Removal of early fruiting branches impacts leaf senescence and yield by altering the sink/source ratio of field-grown cotton. Chen, Yizhen,Dong, Hezhong,Chen, Yizhen,Kong, Xiangqiang,Dong, Hezhong,Kong, Xiangqiang,Dong, Hezhong. 2018

[16]An Improved CTAB-Ammonium Acetate Method for Total RNA Isolation from Cotton. Ding, Qi,Zeng, Jun,He, Xin-Qiang,Zhao, Lu,Fan, Shou-Jin,Wang, Fu-Rong,Zhang, Jun. 2012

[17]Yield, quality and leaf senescence of cotton grown at varying planting dates and plant densities in the Yellow River Valley of China. Dong, HZ,Li, WJ,Tang, W,Li, ZH,Zhang, DM,Niu, YH. 2006

[18]Dry mycelium of Penicillium chrysogenum protects cotton plants against wilt diseases and increases yield under field conditions. Dong, HZ,Zhang, XK,Choen, Y,Zhou, Y,Li, WJ,Li, ZH. 2006

[19]Lint yield and nitrogen use efficiency of field-grown cotton vary with soil salinity and nitrogen application rate. Zhang, Dongmei,Li, Weijiang,Xin, Chengsong,Tang, Wei,Eneji, A. Egrinya,Dong, Hezhong,Eneji, A. Egrinya. 2012

[20]Unequal salt distribution in the root zone increases growth and yield of cotton. Dong, Hehzong,Kong, Xianggiang,Luo, Zhen,Li, Weijiang,Xin, Chengsong. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台