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E-Cadherin Facilitates Protein Kinase D1 Activation and Subcellular Localization

文献类型： 外文期刊

作者： Li, Zhuo 1 ; Zhang, Chuanyou 2 ; Chen, Li 2 ; Li, Guosheng 3 ; Qu, Ling 3 ; Balaji, K. C. 4 ; Du, Cheng 2 ;

作者机构： 1.China Med Univ, Affiliated Hosp 1, Shenyang, Peoples R China

2.Univ Massachusetts, Sch Med, Dept Surg, 364 Plantat St, Worcester, MA 01605 USA

3.Shandong Acad Agr Sci, Jinan, Peoples R China

4.Wake Forest Univ, Dept Urol, Winston Salem, NC 27109 USA

5.Wake Forest Univ, Inst Regenerat Med, Winston Salem, NC 27109 USA

期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）

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收录情况： SCI

摘要： Protein kinase D 1 (PKD1) is a serine/threonine kinase implicated in the regulation of diverse cellular functions including cell growth, differentiation, adhesion and motility. The current model for PKD1 activation involves diacylglycerol (DAG) binding to the C1 domain of PKD1 which results in the translocation of PKD1 to subcellular membranes where PKD1 is phosphorylated and activated by protein kinase C (PKC). In this study, we have identified a novel regulation of PKD1 activation. The epithelial cell membrane protein E-cadherin physically binds to PKD1 which leads to a subcellular redistribution of PKD1. Furthermore, artificial targeting of PKD1 to the membrane leads to PKD1 activation in a PKC-independent manner, indicating that membrane attachment is sufficient enough to activate PKD1. The presence of E-cadherin dynamically regulates PKD1 activation by Bryostatin 1, a potent activator of PKD1, and its substrate phosphorylation specificity, implying a loss of E-cadherin during cancer metastasis could cause the re-distribution PKD1 and re-wiring of PKD1 signaling for distinct functions. The knocking down of PKD1 in lung epithelial cell line A549 results in an epithelial to mesenchymal transition with changes in biomarker expression, cell migration and drug resistance. These results extend our previous understanding of PKD1 regulation and E-cadherin signaling functions and may help to explain the diversified functions of PKD1 in various cells. (C) 2016 Wiley Periodicals, Inc.

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