文献类型： 外文期刊

作者： Wang, H. -M. ¹ ; Xia, X. -Z. ¹ ; Hu, G. -X. ¹ ; Yu, L. ⁴ ; He, H. -B. ¹ ;

作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China

2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250100, Peoples R China

3.Acad Mil Med Sci, Inst Mil Vet, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Peoples R China

4.Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Livestock Infect Dis, Harbin 150001, Peoples R China

关键词： bovine Mx1 gene;foot-and-mouth disease virus;bovine primary fetal tracheal epithelial cell;anti-viral effects

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

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收录情况： SCI

摘要： Innate immunity, especially the anti-viral genes, exerts an important barrier function in preventing viral infections. Myxovirus-resistant (Mx) gene take an anti-viral role, whereas its effects on foot-and-mouth disease virus (FMDV) in naturally susceptible cells are still unclear. The bovine primary fetal tracheal epithelial cell line BPTE-siMx1, in which bovine Mx1 gene was silenced, was established and treated with IFN alpha for 6 hr before FMDV infection. The copy numbers of the negative and positive strand viral RNA were determined by strand-specific real-time fluorescence quantitative RT-PCR. The TCID50 of BPTE-siMx1 cells increased at least 17-fold as compared to control cells BPTE-LacZ at 8 hr post infection, thus silencing of bovine Mx1 could promote the replication of FMDV. The amount of both the negative and positive strand viral RNA in BPTE-siMx1 cells significantly increased as compared to BPTE-LacZ cells, indicating that the replication levels of viral RNA were promoted by silencing bovine Mx1. The bovine Mx1 gene could provide resistance against FMDV in the bovine primary fetal tracheal epithelial cells via suppressing the replication of viral RNA.