Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China
文献类型： 外文期刊
作者： Wang, Jiawei 1 ; Zhu, Dongzi 1 ; Tan, Yue 1 ; Zong, Xiaojuan 1 ; Wei, Hairong 1 ; Hammond, Rosemarie W. 2 ; Liu, Qingz 1 ;
作者机构： 1.Shandong Inst Pomol, Key Lab Fruit Biotechnol Breeding Shandong Prov, Tai An 271000, Shandong, Peoples R China
2.ARS, USDA, Mol Plant Pathol Lab, Room 214,Bldg 004 BARC West, Beltsville, MD 20705 USA
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
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收录情况： SCI
摘要： Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.
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