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A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity purified from the fungus Ramaria formosa

文献类型： 外文期刊

作者： Zhang, Rui 2 ; Tian, Guoting 3 ; Zhao, Yongchang 3 ; Zhao, Liyan 4 ; Wang, Hexiang 1 ; Gong, Zhiyuan 5 ; Ng, Tzi Bun 6 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming, Peoples R China

4.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China

5.Shandong Acad Agr Sci, Inst Agr Resources & Environm, Jinan, Shandong, Peoples R China

6.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China

关键词： Ribonuclease;HIV-1 reverse transcriptase;Ramaria formosa

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

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收录情况： SCI

摘要： A purification protocol that encompassed anion exchange chromatography (EC) on DEAE-cellulose, cation EC on CM-cellulose, anion EC on Q-Sepharose, and fast protein liquid chromatography-gel filtration of Superdex 75 was used to isolate a ribonuclease from dried fruiting bodies of Ramaria formosa. The ribonuclease was unadsorbed on CM-cellulose but adsorbed on both DEAE-cellulose and Q-Sepharose. It displayed a molecular mass of 29-kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ranking of its ribonucleolytic activity toward polyhomoribonucleotides was poly(U)>poly(C)>poly(G)>poly(A). It exhibited a pH optimum of pH 5 and a temperature optimum at 60 degrees C. The ribonuclease inhibited HIV-1 reverse transcriptase with an IC50 of 3 mu M.

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