文献类型： 外文期刊

作者： He, P. ¹ ; Li, L. G. ¹ ; Li, H. F. ¹ ; Wang, H. B. ¹ ; Yang, J. M. ¹ ;

作者机构： 1.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

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收录情况： SCI

摘要： Apple (Malus x domestica Borkh.) is a widely-cultivated, economically important fruit crop with nutritional and medicinal value. Apple has emerged as a model horticultural crop in the post-genomic era. Apple sport resources are important, develop gradually in the apple industry, and provide genetic diversity. In this study, we have successfully developed a sequence-specific amplification polymorphism (S-SAP) marker system based on Ty1-copia retrotransposons in the apple genome. Among the 34 primer combinations used to develop the S-SAP marker system, two primer combinations produced profiles with clear and different peak patterns. Based on these primer combinations, 15 apple sport accessions (four 'Gala', six 'Fuji', and five 'Delicious') were grouped into three clusters based on a genetic variation coefficient value of 0.78. In addition, 27 'Delicious' apple sport accessions were also selected to perform S-SAP marker analysis using the same two primer combinations. The results showed that all 'Delicious' apple sport accessions tested were clearly distinguished. The S-SAP data shared many consistencies with known relationships among apple sport accessions. The available evidence showed that these S-SAP markers had a high level of polymorphism in these apple accessions, which could be applied efficiently to determine genetic diversity, systematic evolution, and for variety identification.