Mutant Rep protein of the porcine circovirus type 2 N-glycosylation:23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication in PK-15 cells
文献类型： 外文期刊
作者： Shi, Jianli 1 ; Peng, Zhe 1 ; Fu, Fang; Xu, Shaojian 1 ; Xu, Shengnan; Cong, Xiaoyan 1 ; Yuan, Xiaoyuan 1 ; Yu, Jia 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Div Swine Dis, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet M
关键词： Porcine circovirus type 2;N-Glycosylation;Mutationat;Infectious clone;Rescued
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN：
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收录情况： SCI
摘要： Porcine circovirus type 2 (PCV2) Rep protein and the splice variant Rep' protein impact genome replication. The Rep protein contains three potential N-glycosylation at positions 23-25aa (NPS), 256-258aa (NQT) and 286-288aa (NAT). Three double copy infectious clones with Rep protein N-glycosylation at positions mutations 23-25aa (DPS), 256-258aa (DQT) and 286-288aa (DAT) were constructed and their function in virus replication in PK-15 cells was investigated. The results showed that the double copy infectious clone with N-glycosylation site mutation could be rescued in vitro and 23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication. (C) 2015 Elsevier B.V. All rights reserved.
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