The construction of a fluorescent nano-probe and its application in detecting transgenic Bt rice TT51-1
文献类型: 外文期刊
作者: Zhang, Guangyuan 1 ; Sun, Hongwei 1 ; Li, Fan 1 ; Yang, Shuke 1 ; Xu, Xiao Hui 1 ; Gao, Rui 1 ; Zhao, Lei 2 ; Lu, Xingbo 1 ;
作者机构: 1.Shandong Acad Agr Sci, Inst Plant Protect, Shandong Key Lab Plant Virol, Jinan, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
期刊名称:RSC ADVANCES ( 影响因子:3.361; 五年影响因子:3.39 )
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收录情况: SCI
摘要: In this study, we used 13 nm gold nanoparticles (AuNPs) as the quenching group and Cy3/Cy5 fluorescent dye as the reporting group to develop a non-PCR method to detect transgenic rice TT51-1. The results showed that it had high sensitivity and specificity, and was time-efficient, inexpensive and simple to use.
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