Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification
文献类型： 外文期刊
作者： Zong, Xiaojuan 1 ; Wang, Wenwen 1 ; Wei, Hairong 1 ; Wang, Jiawei 1 ; Chen, Xin 1 ; Xu, Li 1 ; Zhu, Dongzi 1 ; Tan, Yue 2 ;
作者机构： 1.Shandong Acad Agr Sci, Shandong Inst Pomol, Key Lab Fruit Biotechnol Breeding Shandong Prov, Tai An 271000, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Shandong Inst Pomol, Key Lab Fruit Biotechnol Breeding S
关键词： PNRSV;RT-LAMP;Detection
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
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收录情况： SCI
摘要： Prunus necrotic ringspot virus (PNRSV) has seriously reduced the yield of Prunus species worldwide. In this study, a highly efficient and specific two-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect PNRSV. Total RNA was extracted from sweet cherry leaf samples using a commercial kit based on a magnetic nanoparticle technique. Transcripts were used as the templates for the assay. The results of this assay can be detected using agarose gel electrophoresis or by assessing in-tube fluorescence after adding SYBR Green I. The assay is highly specific for PNRSV, and it is more sensitive than reverse-transcription polymerase chain reaction (RT-PCR). Restriction enzyme digestion verified further the reliability of this RT-LAMP assay. To our knowledge, this is the first report of the application of RT-LAMP to PNRSV detection in Prunus species
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