文献类型： 外文期刊

作者： Sun, H-Z ¹ ; Zhao, S-Z ² ; Ai, H-B ³ ;

作者机构： 1.Qi Lu Normal Univ, Dept Biol, Jinan 250013, Peoples R China

2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Peoples R China

3.Shandong Normal Univ, Coll Life Sci, Key Lab Anim Resistance, Jinan, Peoples R China

关键词： Gastric motility;Hexamethonium bromide;Nitric oxide;Nucleus ambiguus;Sodium nitroprusside;Vagotomy

期刊名称：JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY （ 影响因子：3.011； 五年影响因子：2.927 ）

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收录情况： SCI

摘要： This study aims to investigate whether exogenous nitric oxide (NO) plays a role in controlling gastric motility within the nucleus ambiguus (NA). Experiments were performed on male Wistar rats anaesthetized with chloral hydrate. Alatex balloon, connected to a pressure transducer, was inserted into the pylorus through the fundus for continuous recording of the change of gastric smooth muscle contractile curves. Microinjection of the NO-donor sodium nitroprusside (SNP; 5 nmol) or L-arginine (L-Arg; 5 nmol) into the NA significantly inhibited gastric motility, whereas the treatment of NOsynthase inhibitor N-nitro-L-arginine methylester (L-NAME) increased gastric motility remarkably. The negative effect of SNP or L-Arg on gastric motility was abolished by bilateral subdiaphragmatic vagotomy as well as by intravenous injection of ganglionic blocker, hexamethonium bromide (Hb). These results demonstrated that NO inhibited gastric motility by activating the cholinergic preganglionic neurons in the NA and through the mediation of vagus nerves.