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Substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4 towards galactose, glucose, and their derivatives

文献类型： 外文期刊

作者： Zou, Yang 3 ; Wang, Wenjun 1 ; Cai, Li; Chen, Leilei; Xue, Mengyang 3 ; Zhang, Xiaomei 3 ; Shen, Jie 1 ; Chen, Min 3 ;

作者机构： 1.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China

2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

3.Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Jinan 250100, Shandong, Peoples R China

4.Shandong Univ, Dept Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China

5.Shandong Univ, Dept Chem & Chem Engn, Jinan 250100, Shandong, Peoples

关键词： Enzymatic synthesis;Galactokinase;Streptococcus pneumoniae;Substrate specificity

期刊名称：BIOORGANIC & MEDICINAL CHEMISTRY LETTERS （ 影响因子：2.823； 五年影响因子：2.677 ）

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收录情况： SCI

摘要： Galactokinases (GalKs) have attracted significant research attention for their potential applications in the enzymatic synthesis of unique sugar phosphates. The galactokinase (GalKSpe4) cloned from Streptococcus pneumoniae TIGR4 presents a remarkably broad substrate range including 14 diverse natural and unnatural sugars. TLC and MS studies revealed that GalKSpe4 had relaxed activity towards galactose derivatives with modifications on the C-6, 4- or 2-positions. Additionally, GalKSpe4 can also tolerate glucose while glucose derivatives with modifications on the C-6, 4- or 2-positions were unacceptable. More interestingly, GalKSpe4 can phosphorylate l-mannose in moderate yield (43%), while other l-sugars such as l-Gal cannot be recognized by this enzyme. These results are very significant because there is rarely enzyme reported that can phosphorylate such uncommon substrates as l-mannose.

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[1]Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4. Chen, Min,Chen, Lei-lei,Zou, Yang,Xue, Mengyang,Liang, Min,Jin, Lan,Guan, Wan-yi,Wang, Lei,Liu, Jun,Wang, Peng George,Chen, Min,Chen, Lei-lei,Zou, Yang,Xue, Mengyang,Liang, Min,Jin, Lan,Guan, Wan-yi,Wang, Lei,Liu, Jun,Wang, Peng George,Chen, Lei-lei,Shen, Jie,Wang, Wenjun,Wang, Peng George,Wang, Peng George.

[2]Functional studies of rat galactokinase. Chu, Xiusheng,Li, Nan,Liu, Xiaojun,Li, Ding,Chu, Xiusheng.

[3]Characterization and Mutational Analysis of Two UDP-Galactose 4-Epimerases in Streptococcus pneumoniae TIGR4. Chen, L. L.,Han, D. L.,Zhai, Y. F.,Chen, M.,Chen, L. L.,Wang, J. H.,Wang, Y. F.. 2018

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