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Two enzymatic sources of nitric oxide in different organs of apple plant

文献类型: 外文期刊

作者: Gao, H. J. 1 ; Yang, H. Q. 2 ; Wang, J. Y. 1 ; Wang, J. X. 1 ;

作者机构: 1.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

2.Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China

3.State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

关键词: chemiluminescence;mitochondria;nitric oxide synthase;nitrites.;L-arginine;Internet resource.

期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Nitric oxide production, nitric oxide synthase (NOS) and mitochondrial nitrite-reducing activities in roots, leaves and stems of different developmental stages were investigated, using potted 3-year-old apple (Malus domestica Borkh.) trees. The arginine-dependent NOS activity is sensitive to NOS inhibitor L-NAME and aminoguanidine (AG), with L-NAME being more effective than AG. Endogenous NO production, NOS and mitochondrial nitrite-reducing activities are predominately presented in young leaves and especially in young white roots and young stems. Root and stem mitochondria can reduce nitrite to nitric oxide at the expense of NADH, however, this mitochondrial nitrite-reducing activity is absent in leaves.

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