Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application
文献类型： 外文期刊
作者： Guo, Changying 1 ; Wu, Xia 1 ; Xu, Wei 1 ; Yang, Jinghe 1 ;
作者机构： 1.Shandong Univ, Sch Chem, Minist Educ, Key Lab Colloid & Interface Chem Shandong Univ, Jinan 250100, Peoples R China
2.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China
3.Shandong Acad Agr Sci, Cent Lab, Jinan 250100, Peoples R China
关键词： resonance light scattering;determination;protein;Y3+-TTA-SLS;CAPILLARY-ELECTROPHORESIS;PROTEINS;DNA;GREEN;ASSAY;BLUE;RED
期刊名称：LUMINESCENCE （ 影响因子：2.464； 五年影响因子：1.961 ）
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年卷期：
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收录情况： SCI
摘要： In this paper, a sensitive resonance light scattering(RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y3+-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 X 10(-9)-1.0 X 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6) g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied. Copyright (c) 2008 John Wiley & Sons, Ltd.
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