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Molecular characterization of a novel strain of Bacillus halotolerans protecting wheat from sheath blight disease caused by Rhizoctonia solani Kuhn

文献类型： 外文期刊

作者： Feng, Zhibin 1 ; Xu, Mingzhi 2 ; Yang, Jin 2 ; Zhang, Renhong 2 ; Geng, Zigui 2 ; Mao, Tingting 2 ; Sheng, Yuting 2 ; Wang, Limin 2 ; Zhang, Juan 2 ; Zhang, Hongxia 2 ;

作者机构： 1.Ludong Univ, Coll Life Sci, Yantai, Peoples R China

2.Ludong Univ, Engn Res Inst Agr & Forestry, Yantai, Peoples R China

3.Ludong Univ, Coll Agr, Yantai, Peoples R China

4.Ludong Univ, Key Lab Mol Module Based Breeding High Yield & Abi, Yantai, Peoples R China

5.Shandong Acad Agr Sci, Shandong Inst Sericulture, Yantai, Peoples R China

关键词： Bacillus halotolerans LDFZ001; Rhizoctonia solani Kuhn; antagonistic activity; sheath blight; wheat

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Rhizoctonia solani Kuhn naturally infects and causes Sheath blight disease in cereal crops such as wheat, rice and maize, leading to severe reduction in grain yield and quality. In this work, a new bacterial strain Bacillus halotolerans LDFZ001 showing efficient antagonistic activity against the pathogenic strain Rhizoctonia solani Kuhn sh-1 was isolated. Antagonistic, phylogenetic and whole genome sequencing analyses demonstrate that Bacillus halotolerans LDFZ001 strongly suppressed the growth of Rhizoctonia solani Kuhn sh-1, showed a close evolutionary relationship with B. halotolerans F41-3, and possessed a 3,965,118 bp circular chromosome. Bioinformatic analysis demonstrated that the genome of Bacillus halotolerans LDFZ001 contained ten secondary metabolite biosynthetic gene clusters (BGCs) encoding five non-ribosomal peptide synthases, two polyketide synthase, two terpene synthases and one bacteriocin synthase, and a new kijanimicin biosynthetic gene cluster which might be responsible for the biosynthesis of novel compounds. Gene-editing experiments revealed that functional expression of phosphopantetheinyl transferase (SFP) and major facilitator superfamily (MFS) transporter genes in Bacillus halotolerans LDFZ001 was essential for its antifungal activity against R. solani Kuhn sh-1. Moreover, the existence of two identical chitosanases may also make contribution to the antipathogen activity of Bacillus halotolerans LDFZ001. Our findings will provide fundamental information for the identification and isolation of new sheath blight resistant genes and bacterial strains which have a great potential to be used for the production of bacterial control agents. ImportanceA new Bacillus halotolerans strain Bacillus halotolerans LDFZ001 resistant to sheath blight in wheat is isolated. Bacillus halotolerans LDFZ001 harbors a new kijanimicin biosynthetic gene cluster, and the functional expression of SFP and MFS contribute to its antipathogen ability.

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