文献类型： 外文期刊

作者： Bao, Zhigui ¹ ; Li, Canhui ² ; Li, Guangcun ³ ; Wang, Pei ¹ ; Peng, Zhen ¹ ; Cheng, Lin ¹ ; Li, Hongbo ¹ ; Zhang, Zhiyang ¹ ; Li, Yuying ¹ ; Huang, Wu ¹ ; Ye, Mingwang ² ; Dong, Daofeng ⁵ ; Cheng, Zhukuan ⁶ ; VanderZaag, Peter ⁷ ; Jacobsen, Evert ⁴ ; Bachem, Christian W. B. ⁴ ; Dong, Suomeng ⁸ ; Zhang, Chunzhi ¹ ; Huang, Sanwen ¹ ; Zhou, Qian ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Genome Anal Lab, Guangdong Lab Lingnan Modern Agr,Minist Agr & Rur, Shenzhen 518120, Peoples R China

2.Yunnan Normal Univ, CAAS YNNU YINMORE Joint Acad Potato Sci, Key Lab Potato Biol Yunnan Prov, Kunming 650500, Yunnan, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Tuber & Root Cro, Minist Agr & Rural Affairs, Beijing 100081, Peoples R China

4.Wageningen Univ & Res, Plant Breeding, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

5.Shandong Acad Agr Sci, Vegetable Res Inst, Jinan 250100, Peoples R China

6.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

7.Sunrise Potato Storage Ltd, Alliston, ON L9R 1V3, Canada

8.Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Peoples R China

9.Nanjing Agr Univ, Key Lab Plant Immun, Nanjing 210095, Peoples R China

10.Peng Cheng Lab, Shenzhen 518055, Peoples R China

关键词： potato genome; polyploid genome; haplotype-resolved assembly; haplotype diversity; potato centromere

期刊名称：MOLECULAR PLANT （ 影响因子：21.949； 五年影响因子：19.617 ）

ISSN： 1674-2052

年卷期： 2022 年 15 卷 7 期

页码：

收录情况： SCI

摘要： Potato (Solanum tuberosum) is the most consumed non-cereal food crop. Most commercial potato cultivars are autotetraploids with highly heterozygous genomes, severely hampering genetic analyses and improvement. By leveraging the state-of-the-art sequencing technologies and polyploid graph binning, we achieved a chromosome-scale, haplotype-resolved genome assembly of a cultivated potato, Cooperation-88 (C88). Intra-haplotype comparative analyses revealed extensive sequence and expression differences in this tetraploid genome. We identified haplotype-specific pericentromeres on chromosomes, suggesting a distinct evolutionary trajectory of potato homologous centromeres. Furthermore, we detected double reduction events that are unevenly distributed on haplotypes in 1021 of 1034 selfing progeny, a feature of autopolyploid inheritance. By distinguishing maternal and paternal haplotype sets in C88, we simulated the origin of heterosis in cultivated tetraploid with a survey of 3110 tetra-allelic loci with deleterious mutations, which were masked in the heterozygous condition by two parents. This study provides insights into the genomic architecture of autopolyploids and will guide their breeding.