您好，欢迎访问山东省农业科学院文献资源数据库平台

High level resistance to Turnip mosaic virus in Chinese cabbage (Brassica campestris ssp pekinensis (Lour) Olsson) transformed with the antisense NIb gene using marker-free Agrobacterium tumefaciens infiltration

文献类型： 外文期刊

作者： Yu Zhandong 1 ; Zhao Shuangyi 2 ; He Qiwei 3 ;

作者机构： 1.Shandong Univ, Coll Life Sci, Jinan 250100, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetable Crops, Jinan 250100, Peoples R China

3.Jilin Agr Univ, Coll Hort, Changchun 130118, Jilin, Peoples R China

关键词： TuMV;NIb gene;marker-free;injiltration gene-transformation system;Chinese cabbage;Agrobacterium tumefaciens;TRANSGENIC ARABIDOPSIS;PLANTS;EXPRESSION;THALIANA;RNA

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A TuMV-Nlb gene fragment was amplified from the total RNA of Chinese cabbage leaves infected with TuMV-C-4 by RT-PCR. This fragment was cloned into the marker-free plant binary vector pROK (a derivative of pROK2) in the antisense orientation and transformed into Chinese cabbage [Brassica canipestris ssp. pekinensis (Lour) Olsson] by the Agrobacterium tutnefaciens strain LBA4404 using infiltration genetransformation system. Forty-three transgenic T-0 plants were obtained. PCR, ELISA and Southern blot analysis showed that the TuMV-Nlb gene had integrated into the genome of Chinese cabbage and the heredity of this gene in some lines followed the 3:1 ratio. Both TO and T, transgenic plants showed high levels of resistance to TuMV when artificially inoculated with TuMV. RT-PCR analysis of To transgenic Chinese cabbage revealed the integrated TuMV-Nlb gene can be expressed normally. The infiltration gene-transformation system had proved effective in transformation of Chinese cabbage. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  • 相关文献

[1]Genome-wide identification and analysis of the growth-regulating factor family in Chinese cabbage (Brassica rapa L. ssp pekinensis). Wang, Fengde,Ding, Qian,Li, Jingjuan,Zhang, Yihui,Li, Huayin,Gao, Jianwei,Qiu, Nianwei. 2014

[2]Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China. Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui,Chen, Jia,Shao, Yun-Hua,Li, Xiang-Dong,Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui. 2009

[3]F_(2∶3)家系法鉴定大白菜TuMV分离群体单株抗性. 满卫萍,张志刚,李春民,赵智中,王荣花,刘栓桃,李巧云,王立华. 2019

[4]大白菜抗TuMV分子标记辅助选择技术研究. 李巧云,张志刚,赵智中,刘栓桃,王立华,高会超,李溢真,徐文玲,刘贤娴,刘辰. 2017

[5]利用ELISA方法鉴定大白菜TuMV抗性. 李巧云,张志刚,成文华,赵智中. 2009

[6]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[7]Production of Proinsulin in Marker-Free Transgenic Tobacco Plants Using CRE/loxP System. Zheng, L.,Wang, Y.,Wan, S. B.,Bi, Y. P.,Zheng, L.,Peng, Z. Y.,Wang, Y.,Bian, F.,Wan, S. B.,Bi, Y. P.,Zheng, L.,Peng, Z. Y.,Wang, Y.,Bian, F.,Wan, S. B.,Bi, Y. P.,Jiao, Q. Q.,Qu, S. J.,Wan, S. B.,Bi, Y. P..

[8]RIG-Ⅰ样受体信号传导通路与调控机制研究进展. 盛晓丹,黄迪海,郭卉,刘霞,秦卓明. 2020

[9]槭树叶片总RNA提取技术优化. 尹燕雷,苑兆和,招雪晴,冯立娟,靳启伟. 2013

[10]利用改良的CTAB-LiCl法提取甘薯块根RNA. 侯夫云,赵兵,赵宝杰,王庆美,李爱贤,张海燕,董顺旭,张立明. 2008

[11]Fine mapping of a quantitative trait locus qHD3-1, controlling the heading date, to a 29.5-kb DNA fragment in rice. Wang, W. Y.,Liu, X.,Ding, H. F.,Liu, W.,Yao, F. Y.,Jiang, M. S.,Li, G. X.,Zhu, C. X..

[12]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[13]Genome-Wide Identification and Analysis of the VQ Motif-Containing Protein Family in Chinese Cabbage (Brassica rapa L. ssp Pekinensis). Zhang, Gaoyuan,Zhang, Jiannong,Wang, Fengde,Li, Jingjuan,Ding, Qian,Zhang, Yihui,Li, Huayin,Gao, Jianwei. 2015

[14]High-throughput sequencing discovery of conserved and novel microRNAs in Chinese cabbage (Brassica rapa L. ssp pekinensis). Wang, Fengde,Li, Libin,Liu, Lifeng,Li, Huayin,Zhang, Yihui,Gao, Jianwei,Yao, Yingyin,Ni, Zhongfu. 2012

[15]PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES ANALYSIS OF LATE-BOLTING TRANSGENIC CHINESE CABBAGE (BRASSICA RAPA L. SSP PEKINENSIS). Xia, G.,He, Q.,Zhao, S.. 2015

[16]Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Tong, Haishen,Song, Xiyun. 2011

[17]Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing. Wang, Fengde,Li, Libin,Li, Huayin,Liu, Lifeng,Zhang, Yihui,Gao, Jianwei,Wang, Xiaowu. 2012

[18]Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering. Song, Mei-Fang,Shang, Hong-Zhong,Gu, Hai-Ke,Song, Mei-Fang,Zhang, Shu,Li, Jing-Juan,Gao, Jian-Wei,Song, Mei-Fang,Hou, Pei,Guo, Lin,Su, Liang,Yang, Jian-Ping,Xiao, Yang.

[19]Physiological and Transcriptomic Responses of Chinese Cabbage (Brassica rapa L. ssp Pekinensis) to Salt Stress. Qiu, Nianwei,Li, Jingjuan,Zhang, Yihui,Wang, Fengde,Gao, Jianwei,Qiu, Nianwei,Liu, Qian. 2017

[20]MicroRNA expression analysis of rosette and folding leaves in Chinese cabbage using high-throughput Solexa sequencing. Wang, Fengde,Li, Huayin,Zhang, Yihui,Li, Jingjuan,Li, Libin,Liu, Lifeng,Wang, Lihua,Wang, Cuihua,Gao, Jianwei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台