Absolute quantification of single-base m(6)A methylation in the mammalian transcriptome using GLORI
文献类型： 外文期刊
作者： Liu, Cong 1 ; Sun, Hanxiao 1 ; Yi, Yunpeng 2 ; Shen, Weiguo 2 ; Li, Kai 4 ; Xiao, Ye 4 ; Li, Fei 2 ; Li, Yuchen 4 ; Hou, Yongkang 2 ; Lu, Bo 1 ; Liu, Wenqing 6 ; Meng, Haowei 1 ; Peng, Jinying 1 ; Yi, Chengqi 1 ; Wang, Jing 2 ;
作者机构： 1.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China
3.Shandong Acad Agr Sci, Inst Poultry Sci, Shandong Prov Anim & Poultry Green Hlth Prod Crea, Jinan, Peoples R China
4.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing, Peoples R China
5.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
6.Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China
7.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Beijing, Peoples R China
8.Peking Univ, Dept Chem Biol, Coll Chem & Mol Engn, Beijing, Peoples R China
9.Peking Univ, Synthet & Funct Biomol Ctr, Coll Chem & Mol Engn, Beijing, Peoples R China
期刊名称：NATURE BIOTECHNOLOGY （ 影响因子：68.164； 五年影响因子：60.196 ）
ISSN： 1087-0156
年卷期：
页码：
收录情况： SCI
摘要： N-6-methyladenosine (m(6)A) is the most abundant RNA modification in mammalian cells and the best-studied epitranscriptomic mark. Despite the development of various tools to map m(6)A, a transcriptome-wide method that enables absolute quantification of m(6)A at single-base resolution is lacking. Here we use glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) to develop an absolute m(6)A quantification method that is conceptually similar to bisulfite-sequencing-based quantification of DNA 5-methylcytosine. We apply GLORI to quantify the m(6)A methylomes of mouse and human cells and reveal clustered m(6)A modifications with differential distribution and stoichiometry. In addition, we characterize m(6)A dynamics under stress and examine the quantitative landscape of m(6)A modification in gene expression regulation. GLORI is an unbiased, convenient method for the absolute quantification of the m(6)A methylome. The m(6)A modification is mapped transcriptome-wide at single-base resolution in mammalian cells.
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