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Functional characterization of DiMMS21, a SUMO ligase from Desmodium intortum

文献类型： 外文期刊

作者： Zhou, Xuan 1 ; Du, Jinju 1 ; Liu, Yiyang 1 ; Yang, Chengwei 1 ; Lai, Jianbin 1 ;

作者机构： 1.South China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China

2.Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Shandong, Peoples R China

3.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China

关键词： Desmodium intortum; MMS21; SUMO ligase; SUMOylation

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2019 年 141 卷

页码：

收录情况： SCI

摘要： SUMOylation is an important protein modification that regulates the properties of substrate proteins in a variety of cellular processes. SUMOylation is catalyzed via a cascade of enzymes and is usually stimulated by SUMO E3 ligases. However, the molecular functions and regulatory mechanisms of SUMOylation in forage crops are unknown. Here, we isolated and functionally characterized DiMMS21, a homolog of the Arabidopsis thaliana SUMO ligase AtMMS21, from the forage legume Desmodium intortum. DiMMS21 is expressed ubiquitously in various D. intortum organs and its encoded protein is found in the cytoplasm and nucleus. Bioinformatics analysis indicated that DiMMS21 contains a conserved SP-RING domain that is required for its activity. Biochemical evidence supports the notion that this protein is a functional SUMO ligase. When expressed in an Arabidopsis rrans21 mutant, DiMMS21 completely rescued the defects in root, leaf, and silique development. The results from cotyledon greening and marker gene expression suggested that DiMMS21 can only partially complements the role of AtMMS21 in abscisic acid (ABA) responses. In summary, we characterized the molecular features of DiMMS21 and uncovered potential roles of this SUMO ligase in development and ABA responses, increasing our understanding on the function of SUMOylation in forage crops.

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[1]Functional characterization of DiMMS21, a SUMO ligase from Desmodium intortum. Zhou, Xuan,Du, Jinju,Liu, Yiyang,Yang, Chengwei,Lai, Jianbin,Liu, Yiyang,Liu, Yiyang. 2019

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